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AB231614

Anti-A1CF/ACF antibody

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(1 Publication)

Rabbit Polyclonal A1CF/ACF antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human A1CF aa 350 to C-terminus.

View Alternative Names

ACF, ASP, A1CF, APOBEC1 complementation factor, APOBEC1-stimulating protein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A1CF/ACF antibody (AB231614)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A1CF/ACF antibody (AB231614)

Formalin-fixed, paraffin-embedded human stomach tissue stained for A1CF/ACF with ab231614 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A1CF/ACF antibody (AB231614)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A1CF/ACF antibody (AB231614)

Formalin-fixed, paraffin-embedded human stomach tissue stained for A1CF/ACF with ab231614 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-A1CF/ACF antibody (AB231614)
  • WB

Supplier Data

Western blot - Anti-A1CF/ACF antibody (AB231614)

All lanes:

Western blot - Anti-A1CF/ACF antibody (ab231614) at 3 µg/mL

Lane 1:

Pig liver tissue lysate

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 289 kDa,65 kDa

Observed band size: 289 kDa

false

Western blot - Anti-A1CF/ACF antibody (AB231614)
  • WB

Supplier Data

Western blot - Anti-A1CF/ACF antibody (AB231614)

All lanes:

Western blot - Anti-A1CF/ACF antibody (ab231614) at 2 µg/mL

All lanes:

Recombinant human A1CF/ACF protein

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human A1CF aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NQ94

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

A1CF also known as APOBEC1 complementation factor is an auxiliary protein that assists in RNA editing. It works with the APOBEC1 enzyme to deaminate cytidines in RNA transcripts converting cytidine to uridine. A1CF has a molecular weight of around 65 kDa. The protein expresses mainly in the liver and small intestine but you can find it in lower levels in other tissues.
Biological function summary

A1CF supports the RNA editing process affecting mRNA transcripts including those involved in lipid metabolism. It contributes to the editing of apolipoprotein B (ApoB) mRNA which results in two different forms of ApoB protein. Although A1CF does not form part of a large stable complex it interacts specifically with APOBEC1 to exert its functions in the cell.

Pathways

A1CF plays a significant role in lipid metabolism especially in the regulation of cholesterol and triglyceride levels. The connection between A1CF and the ApoB mRNA editing pathway illustrates its importance in lipid transport and energy homeostasis. This pathway involves interactions with other proteins such as APOBEC1 and components of the lipid transport system emphasizing A1CF’s function in modulating lipid profiles.

A1CF's role in lipid metabolism implicates it in metabolic conditions such as hyperlipidemia and non-alcoholic fatty liver disease (NAFLD). Abnormalities in A1CF function can lead to improper ApoB mRNA editing which influences lipid levels and contributes to these diseases. Additionally A1CF connections with APOBEC1 and the lipid regulation pathway highlight its potential impact in modulating disease outcomes through RNA editing errors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
See full target information A1CF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed35884490

2022

Ras-Related Protein Rab-32 and Thrombospondin 1 Confer Resistance to the EGFR Tyrosine Kinase Inhibitor Osimertinib by Activating Focal Adhesion Kinase in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zeinab Kosibaty,Odd Terje Brustugun,Inger Johanne Zwicky Eide,Georgios Tsakonas,Oscar Grundberg,Luigi De Petris,Marc McGowan,Per Hydbring,Simon Ekman
View all publications
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