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AB89050

Anti-A1CF/ACF antibody

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(2 Publications)

Mouse Polyclonal A1CF/ACF antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human A1CF.

View Alternative Names

ACF, ASP, A1CF, APOBEC1 complementation factor, APOBEC1-stimulating protein

3 Images
Western blot - Anti-A1CF/ACF antibody (AB89050)
  • WB

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Western blot - Anti-A1CF/ACF antibody (AB89050)

All lanes:

Western blot - Anti-A1CF/ACF antibody (ab89050) at 1 µg/mL

All lanes:

Human placenta lysate at 50 µg

Predicted band size: 65 kDa

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Western blot - Anti-A1CF/ACF antibody (AB89050)
  • WB

Unknown

Western blot - Anti-A1CF/ACF antibody (AB89050)

All lanes:

Western blot - Anti-A1CF/ACF antibody (ab89050) at 1 µg/mL

Lane 1:

A1CF/ACF-transfected 293T cell lysate at 25 µg

Lane 2:

non transfected lysate at 25 µg

Predicted band size: 65 kDa

Observed band size: 65 kDa

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Western blot - Anti-A1CF/ACF antibody (AB89050)
  • WB

Unknown

Western blot - Anti-A1CF/ACF antibody (AB89050)

All lanes:

Western blot - Anti-A1CF/ACF antibody (ab89050) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 65 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human A1CF.

Q9NQ94

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A1CF/ACF antibody (AB231614)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

A1CF also known as APOBEC1 complementation factor is an auxiliary protein that assists in RNA editing. It works with the APOBEC1 enzyme to deaminate cytidines in RNA transcripts converting cytidine to uridine. A1CF has a molecular weight of around 65 kDa. The protein expresses mainly in the liver and small intestine but you can find it in lower levels in other tissues.
Biological function summary

A1CF supports the RNA editing process affecting mRNA transcripts including those involved in lipid metabolism. It contributes to the editing of apolipoprotein B (ApoB) mRNA which results in two different forms of ApoB protein. Although A1CF does not form part of a large stable complex it interacts specifically with APOBEC1 to exert its functions in the cell.

Pathways

A1CF plays a significant role in lipid metabolism especially in the regulation of cholesterol and triglyceride levels. The connection between A1CF and the ApoB mRNA editing pathway illustrates its importance in lipid transport and energy homeostasis. This pathway involves interactions with other proteins such as APOBEC1 and components of the lipid transport system emphasizing A1CF’s function in modulating lipid profiles.

A1CF's role in lipid metabolism implicates it in metabolic conditions such as hyperlipidemia and non-alcoholic fatty liver disease (NAFLD). Abnormalities in A1CF function can lead to improper ApoB mRNA editing which influences lipid levels and contributes to these diseases. Additionally A1CF connections with APOBEC1 and the lipid regulation pathway highlight its potential impact in modulating disease outcomes through RNA editing errors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
See full target information A1CF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer biotherapy & radiopharmaceuticals : PubMed32818382

2020

High APOBEC1 Complementation Factor Expression Positively Modulates the Proliferation, Invasion, and Migration of Endometrial Cancer Cells Through Regulating P53/P21 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Liu,Chun-Yan Chen,Gui-Lin Chen

EMBO reports 15:903-10 PubMed24916387

2014

C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Fossat,Karin Tourle,Tania Radziewic,Kristen Barratt,Doreen Liebhold,Joshua B Studdert,Melinda Power,Vanessa Jones,David A F Loebel,Patrick P L Tam
View all publications
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Product promise

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