JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183348

Anti-A2BP1/Fox1/RBFOX1 antibody [1G10]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal A2BP1/Fox1/RBFOX1 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBFOX1.

View Alternative Names

A2BP, A2BP1, FOX1, HRNBP1, RBFOX1, RNA binding protein fox-1 homolog 1, Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide-binding protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [1G10] (AB183348)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [1G10] (AB183348)

Immunofluorescent analysis of cultured Rat neurons labeling A2BP1/Fox1/RBFOX1 with ab183348 at 1/500 dilution.

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [1G10] (AB183348)
  • WB

Supplier Data

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [1G10] (AB183348)

All lanes:

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [1G10] (ab183348) at 1/5000 dilution

All lanes:

Rat cerebral cortex tissue lysate

Predicted band size: 42 kDa

Observed band size: 46-48 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1G10

Isotype

IgG1

Carrier free

No

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RBFOX1. The exact immunogen used to generate this antibody is proprietary information.

Q9NWB1

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.065% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The A2BP1 protein also known as Fox1 or RBFOX1 is a splicing factor that serves an important role in alternative RNA splicing. The protein has a mass of approximately 43 kDa and prominently expresses in the brain muscle and heart. A2BP1/Fox1 is recognized for its RNA-binding capabilities specifically targeting the (U)GCAUG sequence which facilitates the regulation of exon inclusion or skipping during mRNA processing.
Biological function summary

A2BP1/Fox1 influences neuronal and muscle development by managing the splicing of important pre-mRNAs. It operates as part of a larger splicing complex collaborating with other RNA-binding proteins to ensure the proper expression of gene transcripts. This control is vital for neuron-specific and muscle-specific exon recognition affecting the functional states of cells in these tissues.

Pathways

A2BP1/Fox1 activity is integral to the nervous system and muscle development pathways. In the nervous system it links closely with proteins like CELF and PTBP1 modulating the splicing of transcripts involved in synaptic functions. Similarly in muscle development A2BP1/Fox1 works with splicing factors within the pathways that direct muscle differentiation and function such as those involving the protein myogenin.

A2BP1/Fox1 has connections to neurological conditions like autism spectrum disorders (ASD) and epilepsy. Altered expression or mutations in the RBFOX1 gene have associations with these conditions impacting neural connectivity and function. In epilepsy A2BP1/Fox1 appears linked with synaptic proteins which when improperly spliced can disrupt normal neuronal signaling contributing to the disorder's pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
See full target information RBFOX1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 4: PubMed30730308

2019

Distinct pathological signatures in human cellular models of myotonic dystrophy subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Ellis Y Kim,David Y Barefield,Andy H Vo,Anthony M Gacita,Emma J Schuster,Eugene J Wyatt,Janel L Davis,Biqin Dong,Cheng Sun,Patrick Page,Lisa Dellefave-Castillo,Alexis Demonbreun,Hao F Zhang,Elizabeth M McNally
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB243727

Anti-A2BP1/Fox1/RBFOX1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB108319

Anti-BDNF antibody [EPR1292]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-BDNF antibody [EPR1292] (AB108319)

  • 4
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB214821

Anti-BMP2 antibody [EPR20807]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (AB214821)

  • 0
  • 0
Primary Antibodies

AB178869

Anti-Oct-1 antibody [EPR16570]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (AB178869)

  • 5
  • 6

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com