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AB243727

Anti-A2BP1/Fox1/RBFOX1 antibody

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(1 Publication)

Rabbit Polyclonal A2BP1/Fox1/RBFOX1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBFOX1 aa 1-150.

View Alternative Names

A2BP, A2BP1, FOX1, HRNBP1, RBFOX1, RNA binding protein fox-1 homolog 1, Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide-binding protein 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for A2BP1/Fox1/RBFOX1 using ab243727 at 1/50 dilution in immunohistochemical analysis.

Low expression as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for A2BP1/Fox1/RBFOX1 using ab243727 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)
  • WB

Unknown

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)

All lanes:

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody (ab243727) at 0.4 µg/mL

All lanes:

Human cerebral cortex tissue

Predicted band size: 42 kDa,61 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody (AB243727)

Paraformaldehyde-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for A2BP1/Fox1/RBFOX1 using ab243727 at 4 μg/mL in ICC/IF analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RBFOX1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9NWB1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The A2BP1 protein also known as Fox1 or RBFOX1 is a splicing factor that serves an important role in alternative RNA splicing. The protein has a mass of approximately 43 kDa and prominently expresses in the brain muscle and heart. A2BP1/Fox1 is recognized for its RNA-binding capabilities specifically targeting the (U)GCAUG sequence which facilitates the regulation of exon inclusion or skipping during mRNA processing.
Biological function summary

A2BP1/Fox1 influences neuronal and muscle development by managing the splicing of important pre-mRNAs. It operates as part of a larger splicing complex collaborating with other RNA-binding proteins to ensure the proper expression of gene transcripts. This control is vital for neuron-specific and muscle-specific exon recognition affecting the functional states of cells in these tissues.

Pathways

A2BP1/Fox1 activity is integral to the nervous system and muscle development pathways. In the nervous system it links closely with proteins like CELF and PTBP1 modulating the splicing of transcripts involved in synaptic functions. Similarly in muscle development A2BP1/Fox1 works with splicing factors within the pathways that direct muscle differentiation and function such as those involving the protein myogenin.

A2BP1/Fox1 has connections to neurological conditions like autism spectrum disorders (ASD) and epilepsy. Altered expression or mutations in the RBFOX1 gene have associations with these conditions impacting neural connectivity and function. In epilepsy A2BP1/Fox1 appears linked with synaptic proteins which when improperly spliced can disrupt normal neuronal signaling contributing to the disorder's pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
See full target information RBFOX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 11:84 PubMed37217978

2023

Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS function.

Applications

Unspecified application

Species

Unspecified reactive species

Luise A Seeker,Nadine Bestard-Cuche,Sarah Jäkel,Nina-Lydia Kazakou,Sunniva M K Bøstrand,Laura J Wagstaff,Justyna Cholewa-Waclaw,Alastair M Kilpatrick,David Van Bruggen,Mukund Kabbe,Fabio Baldivia Pohl,Zahra Moslehi,Neil C Henderson,Catalina A Vallejos,Gioele La Manno,Goncalo Castelo-Branco,Anna Williams
View all publications
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