Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skeletal muscle. The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on human liver (PMID : 10814712). The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human brain. The section was incubated with ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Dystrophin (ab275391, red; Opal™690), anti-A2BP1/Fox1/RBFOX1 (ab254413, green; Opal™520) and anti-Creatine Kinase MM (ab283683, magenta; Opal™570) on human skeletal muscle. Panel B : anti-A2BP1/Fox1/RBFOX1 stained on nucleus of skeletal muscle. Panel C : anti-Creatine Kinase MM stained on cytoplasm of skeletal muscle. Panel D : anti-Dystrophin stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab275391 at 1/500 (2.08 μg/ml) dilution, ab254413 at 1/2000 (0.239 μg/ml) dilution, and ab283683 at 1/3000 (0.18 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/50 (9.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control : no staining on rat liver (PMID : 16260614) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on mouse liver. The section was incubated with ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/50 (9.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/1000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control : no staining on mouse liver (PMID : 16260614) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Slow Skeletal Myosin Heavy chain with ab234431 at a 1/2500 dilution, A2BP1 with ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

Panel A : merged staining of anti-Slow Skeletal Myosin Heavy chain (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B : anti-Slow Skeletal Myosin Heavy chain staining slow type fibers in mouse skeletal muscle.
Panel C : anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D : anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab234431, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling A2BP1/Fox1/RBFOX1 with ab254413 at 1/50 (9.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum. The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on rat liver. The section was incubated with ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254413 at 1/100 (4.78 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cardiac muscle tissue staining Nav1.5 with ab300048 at a 1/1000 (5.12 µg/ml) dilution, A2BP1 with ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

Panel A : merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse cardiac muscle.
Panel B : anti-Nav1.5 staining cytoplasm in mouse cardiac muscle.
Panel C : anti-A2BP1 staining nucleus in mouse cardiac muscle.
Panel D : anti-Dystrophin staining membrane in mouse cardiac muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab300048, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining SERCA1 ATPase with ab314660 at a 1/5000 (0.103 ug/ml) dilution, A2BP1 with ab254413 at 1/2000 (0.26 ug/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 ug/ml) dilution.

Panel A : merged staining of anti-SERCA1 ATPase (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B : anti-SERCA1 ATPase staining cytoplasm in mouse skeletal muscle.
Panel C : anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D : anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314660, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Nav1.5 with ab300048 at a 1/1000 (5.12 µg/ml) dilution, A2BP1 with ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

Panel A : merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B : anti-Nav1.5 staining cytoplasm in mouse skeletal muscle.
Panel C : anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D : anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab300048, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (AB254413)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : liver (PMID : 12574126, PMID : 16260614, PMID : 20724578, PMID : 20724578, PMID : 10814712).

Exposure time : 37 seconds.

All lanes:

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (ab254413) at 1/1000 dilution

Lane 1:

Mouse brain cortex tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat brain cortex tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 43 kDa

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