Rabbit Recombinant Monoclonal A2BP1/Fox1/RBFOX1 antibody. Carrier free. Suitable for mIHC, ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62167.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29429.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527)")
![Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--western-blot-img16760.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116812.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527)")
![Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-frozen-sections-img68093.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (AB278527)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | ICC/IF | IP | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested | Expected | Not recommended |
| Mouse | Tested | Tested | Not recommended | Tested | Tested | Tested | Tested |
| Rat | Expected | Expected | Not recommended | Tested | Tested | Expected | Expected |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
Alternative names
A2BP, A2BP1, FOX1, HRNBP1, RBFOX1, RNA binding protein fox-1 homolog 1, Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide-binding protein 1
Recommended products
Rabbit Recombinant Monoclonal A2BP1/Fox1/RBFOX1 antibody. Carrier free. Suitable for mIHC, ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23656-5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab278527 is the carrier-free version of Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The A2BP1 protein also known as Fox1 or RBFOX1 is a splicing factor that serves an important role in alternative RNA splicing. The protein has a mass of approximately 43 kDa and prominently expresses in the brain muscle and heart. A2BP1/Fox1 is recognized for its RNA-binding capabilities specifically targeting the (U)GCAUG sequence which facilitates the regulation of exon inclusion or skipping during mRNA processing.
- Biological function summary
A2BP1/Fox1 influences neuronal and muscle development by managing the splicing of important pre-mRNAs. It operates as part of a larger splicing complex collaborating with other RNA-binding proteins to ensure the proper expression of gene transcripts. This control is vital for neuron-specific and muscle-specific exon recognition affecting the functional states of cells in these tissues.
- Pathways
A2BP1/Fox1 activity is integral to the nervous system and muscle development pathways. In the nervous system it links closely with proteins like CELF and PTBP1 modulating the splicing of transcripts involved in synaptic functions. Similarly in muscle development A2BP1/Fox1 works with splicing factors within the pathways that direct muscle differentiation and function such as those involving the protein myogenin.
- Associated diseases and disorders
A2BP1/Fox1 has connections to neurological conditions like autism spectrum disorders (ASD) and epilepsy. Altered expression or mutations in the RBFOX1 gene have associations with these conditions impacting neural connectivity and function. In epilepsy A2BP1/Fox1 appears linked with synaptic proteins which when improperly spliced can disrupt normal neuronal signaling contributing to the disorder's pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
21 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62167.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human skeletal muscle. The section was incubated with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29429.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human brain. The section was incubated with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--western-blot-img16760.jpg "Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver (PMID:12574126, PMID:16260614, PMID:20724578, PMID:20724578, PMID:10814712).
Exposure time: 37 seconds.
All lanes: Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] (Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413) at 1/1000 dilution
Lane 1: Mouse brain cortex tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
Lane 4: Mouse liver tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat brain cortex tissue lysate at 20 µg
Lane 7: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116812.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum. The section was incubated with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-frozen-sections-img68093.jpg "Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/100 (4.78 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunocytochemistry-immunofluorescence-img95735.jpg "Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/50 (9.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunocytochemistry-immunofluorescence-img98453.jpg "Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/50 (9.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Neuro-2a cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Flow Cytometry (Intracellular) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--flow-cytometry-intracellular-img27213.jpg "Flow Cytometry (Intracellular) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Flow Cytometry (Intracellular) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/1000 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img11283.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum. The section was incubated with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-frozen-sections-img122098.jpg "Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/100 (4.78 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunocytochemistry-immunofluorescence-img95115.jpg "Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/50 (9.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-frozen-sections-img106292.jpg "Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/100 (4.78 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on mouse liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-frozen-sections-img101845.jpg "Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/100 (4.78 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on rat liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118144.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on human liver (PMID:10814712). The section was incubated with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109154.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse liver. The section was incubated with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28096.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on rat liver. The section was incubated with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--multiplex-immunohistochemistry-img420904.jpg "Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Dystrophin (Anti-Dystrophin antibody [EPR23336-129] ab275391, red; Opal™690), anti-A2BP1/Fox1/RBFOX1 (Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, green; Opal™520) and anti-Creatine Kinase MM (Anti-Creatine Kinase MM antibody [EPR25358-10] ab283683, magenta; Opal™570) on human skeletal muscle. Panel B: anti-A2BP1/Fox1/RBFOX1 stained on nucleus of skeletal muscle. Panel C: anti-Creatine Kinase MM stained on cytoplasm of skeletal muscle. Panel D: anti-Dystrophin stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of Anti-Dystrophin antibody [EPR23336-129] ab275391 at 1/500 (2.08 μg/ml) dilution, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.239 μg/ml) dilution, and Anti-Creatine Kinase MM antibody [EPR25358-10] ab283683 at 1/3000 (0.18 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.![Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--multiplex-immunohistochemistry-img438268.jpg "Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cardiac muscle tissue staining Nav1.5 with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at a 1/1000 (5.12 µg/ml), dilution A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.
Panel A: merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse cardiac muscle.
Panel B: anti-Nav1.5 staining cytoplasm in mouse cardiac muscle.
Panel C: anti-A2BP1 staining nucleus in mouse cardiac muscle.
Panel D: anti-Dystrophin staining membrane in mouse cardiac muscle.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
![Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--multiplex-immunohistochemistry-img438269.jpg "Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Nav1.5 with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at a 1/1000 (5.12 µg/ml) dilution, A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.
Panel A: merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B: anti-Nav1.5 staining cytoplasm in mouse skeletal muscle.
Panel C: anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D: anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
![Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--multiplex-immunohistochemistry-img438270.jpg "Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at a 1/5000 (0.103 ug/ml) dilution, A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 ug/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 ug/ml) dilution.
Panel A: merged staining of anti-SERCA1 ATPase (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B: anti-SERCA1 ATPase staining cytoplasm in mouse skeletal muscle.
Panel C: anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D: anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--multiplex-immunohistochemistry-img438331.jpg "Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
Multiplex immunohistochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)
This data was developed using Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Slow Skeletal Myosin Heavy chain with Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] ab234431 at a 1/2500 dilution, A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.
Panel A: merged staining of anti-Slow Skeletal Myosin Heavy chain (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B: anti-Slow Skeletal Myosin Heavy chain staining slow type fibers in mouse skeletal muscle.
Panel C: anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D: anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] ab234431, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunocytochemistry-immunofluorescence-img95735.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
![Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunocytochemistry-immunofluorescence-img98453.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
![Flow Cytometry (Intracellular) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--flow-cytometry-intracellular-img27213.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img11283.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
![Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunohistochemistry-frozen-sections-img122098.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
![Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527), expandable thumbnail](https://content.abcam.com/products/images/a2bp1-fox1-rbfox1-antibody-epr23656-5-bsa-and-azide-free-ab278527--immunocytochemistry-immunofluorescence-img95115.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] - BSA and Azide free (ab278527)")
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