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AB73055

Anti-A3H antibody

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(1 Publication)

Mouse Polyclonal A3H antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human APOBEC3H.

View Alternative Names

DNA dC->dU-editing enzyme APOBEC-3H, APOBEC-related protein 10, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H, ARP-10, A3H, APOBEC3H

2 Images
Western blot - Anti-A3H antibody (AB73055)
  • WB

Unknown

Western blot - Anti-A3H antibody (AB73055)

All lanes:

Western blot - Anti-A3H antibody (ab73055) at 1/500 dilution

All lanes:

human colon tissue lysate at 25 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

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Western blot - Anti-A3H antibody (AB73055)
  • WB

Unknown

Western blot - Anti-A3H antibody (AB73055)

All lanes:

Western blot - Anti-A3H antibody (ab73055) at 1/500 dilution

Lane 1:

A3H transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 24 kDa

Observed band size: 19 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human APOBEC3H.

Q6NTF7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p>Expected band of 21.5 kDa (Isoform 2).</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: Whole serum, 0.812% Sodium chloride, 0.1136% Disodium hydrogenorthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

A3H also known as APOBEC3H is a member of the apolipoprotein B mRNA editing enzyme catalytic polypeptide-like (APOBEC) family. The protein has a molecular mass of approximately 23 kDa. A3H is mainly expressed in immune-related tissues such as lymph nodes and spleen and plays a role in the innate immune response. It functions as a cytidine deaminase converting cytidine to uridine in single-stranded DNA which can lead to mutations in viral genomes.
Biological function summary

A3H plays a role in the defense against viral pathogens by editing viral genomes and inhibiting their replication. A3H exists as part of a cellular defense complex that acts against retroviruses such as HIV-1. By inducing hypermutation A3H restricts viral replication and spread. Among the APOBEC3 family members A3H shows unique substrate specificity and activity profiles contributing to the diversity of the host antiviral response.

Pathways

A3H integrates into the broader context of innate immune pathways including interferon signaling. In these pathways A3H serves as a critical effector in innate viral immunity complementing the action of other APOBEC3 family members like APOBEC3G. These proteins work together to inhibit the replication of retroviruses through distinct yet overlapping pathways contributing to a robust antiviral state within host cells.

A3H links to HIV-1 pathogenesis and can influence disease progression. It plays a pivotal role in restricting HIV-1 by incorporating itself into viral particles and inducing detrimental mutations. This activity can affect HIV-1 resistance to therapy. Other APOBEC proteins such as APOBEC3G also participate in this process reflecting a coordinated response that counters HIV-1 infection and affects virus-host interactions. Understanding A3H functions and interactions contributes to better insights into viral pathogenesis and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms (PubMed : 16571802, PubMed : 16920826, PubMed : 18779051, PubMed : 18827027, PubMed : 20062055, PubMed : 22915799, PubMed : 29290613). The A3H-var/haplotype 2 exhibits antiviral activity against vif-deficient HIV-1 (PubMed : 18299330, PubMed : 21835787, PubMed : 23097438, PubMed : 29290613). After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA (PubMed : 18299330). The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells (PubMed : 18299330). Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA (PubMed : 20062055). Exhibits antiviral activity also against T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons (PubMed : 20062055, PubMed : 22457529).
See full target information APOBEC3H
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 44:7848-65 PubMed27439715

2016

APOBEC3 proteins can copackage and comutate HIV-1 genomes.

Applications

Unspecified application

Species

Unspecified reactive species

Belete A Desimmie,Ryan C Burdick,Taisuke Izumi,Hibiki Doi,Wei Shao,W Gregory Alvord,Kei Sato,Yoshio Koyanagi,Sara Jones,Eleanor Wilson,Shawn Hill,Frank Maldarelli,Wei-Shau Hu,Vinay K Pathak
View all publications
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