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AB237802

Anti-AACS antibody

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(2 Publications)

Rabbit Polyclonal AACS antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AACS aa 400-600.

View Alternative Names

ACSF1, AACS, Acetoacetyl-CoA synthetase, Acyl-CoA synthetase family member 1, Protein sur-5 homolog

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACS antibody (AB237802)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACS antibody (AB237802)

Paraffin-embedded human kidney tissue stained for AACS using ab237802 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-AACS antibody (AB237802)
  • WB

Supplier Data

Western blot - Anti-AACS antibody (AB237802)

All lanes:

Western blot - Anti-AACS antibody (ab237802) at 1/500 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human AACS aa 400-600. The exact immunogen used to generate this antibody is proprietary information.

Q86V21

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AACS also known as acetoacetyl-CoA synthetase plays a role in ketone body metabolism. This enzyme has a molecular mass of about 74 kDa and facilitates the conversion of acetoacetate into acetoacetyl-CoA. AACS expression is noticeable in tissues like the liver and brain where high energy demand exists. By helping in ketone body utilization AACS provides an important link in energy metabolism pathways.
Biological function summary

Acetoacetyl-CoA synthetase contributes significantly to lipid synthesis and energy production processes. It activates acetoacetate to form acetoacetyl-CoA an essential intermediate in cholesterol and fatty acid synthesis. Acetoacetyl-CoA plays an important role acting in sequential steps with acetyl-CoA and other enzymes to form more complex lipid molecules although the AACS enzyme does not form a complex itself.

Pathways

The role of AACS connects particularly to the ketone body and the acetyl-CoA biosynthetic pathways. AACS facilitates acetoacetate utilization in the aforementioned pathways enabling energy generation alongside enzymes such as HMG-CoA synthase and fatty acid synthase. The interaction among these enzymes highlights the integration of ketone metabolism within broader metabolic networks.

Faulty AACS functions have links to metabolic syndromes like diabetes and neurological disorders such as Alzheimer's disease. In diabetes improper ketone body utilization can lead to metabolic imbalance potentially involving enzymes like insulin receptor kinases. Within Alzheimer's disease deviances in lipid synthesis pathways where AACS is involved can contribute to disease progression due to altered energy metabolism in brain tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts acetoacetate to acetoacetyl-CoA in the cytosol (By similarity). Ketone body-utilizing enzyme, responsible for the synthesis of cholesterol and fatty acids (By similarity).
See full target information AACS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:5 PubMed38169393

2024

PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Tan,Qi Pan,Chao Yu,Xinyu Zhai,Jianyi Gu,Le Tao,Dongliang Xu

Cell & bioscience 13:45 PubMed36879298

2023

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,Wei Dong,Menghao Zhou,Chenlu Yang,Ming Xiong,Gallina Kazobinka,Zhaohui Chen,Yifei Xing,Teng Hou
View all publications
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Product promise

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