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AB201987

Anti-AACT antibody [5G3C11] - C-terminal

1

(1 Review)

|

(1 Publication)

Mouse Monoclonal AACT antibody. C-terminal. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINA3 aa 250 to C-terminus.

View Alternative Names

AACT, GIG24, GIG25, SERPINA3, Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling AACT with ab201987 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling AACT with ab201987 at 1/200 dilution.

Flow Cytometry - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)

Flow cytometric analysis of A549 cells using ab201987 at 1/200 dilution (green) and negative control (red).

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)
  • WB

Supplier Data

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)

All lanes:

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (ab201987) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

AACT (AA: 279-432)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 47 kDa

false

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)
  • WB

Supplier Data

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)

All lanes:

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (ab201987) at 1/500 dilution

All lanes:

A549 cell lysate

Predicted band size: 47 kDa

false

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)
  • WB

Supplier Data

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (AB201987)

All lanes:

Western blot - Anti-AACT antibody [5G3C11] - C-terminal (ab201987) at 1/500 dilution

All lanes:

AACT (AA: 279-432) recombinant protein

Predicted band size: 47 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G3C11

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human SERPINA3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01011

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Orangutan": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein Alpha-1-antichymotrypsin (AACT) also known as ACT is a serpin family member with a mass of approximately 68 kDa. This protein functions as an inhibitor of serine proteases particularly targeting chymotrypsin-like enzymes. AACT is expressed in the liver and secreted into the plasma where it carries out its inhibitory role. It is a significant component of the antiprotease shield in human physiology guarding tissues against proteolytic damage.
Biological function summary

Alpha-1-antichymotrypsin plays a role in regulating protease activity to maintain tissue integrity. AACT forms part of a complex that involves other protease inhibitors and enzymes contributing to the balance of protein breakdown and regeneration. Its presence in the extracellular matrix highlights its structural and protective functions supporting cells against enzyme-mediated degradation.

Pathways

Alpha-1-antichymotrypsin takes part in inflammatory and immune response pathways. It interacts within the acute phase response pathway a system activated during inflammatory events. This protein's relationship with other protease inhibitors such as alpha-1-antitrypsin accentuates its role in controlling proteolytic enzymes that could otherwise propagate unchecked inflammation and tissue damage.

Alpha-1-antichymotrypsin associates with neurodegenerative diseases such as Alzheimer's disease and pulmonary disorders. Its elevated levels have been observed in Alzheimer's disease cases where it might participate alongside amyloid-beta precursor protein influencing amyloid plaque formation. In lung-related disorders its collaboration with proteins like elastase can affect tissue breakdown emphasizing its role in maintaining pulmonary health.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
See full target information SERPINA3

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:6839 PubMed34824237

2021

Cell-fate transition and determination analysis of mouse male germ cells throughout development.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexiang Zhao,Ping Lu,Cong Wan,Yaping Huang,Manman Cui,Xinyan Yang,Yuqiong Hu,Yi Zheng,Ji Dong,Mei Wang,Shu Zhang,Zhaoting Liu,Shuhui Bian,Xiaoman Wang,Rui Wang,Shaofang Ren,Dazhuang Wang,Zhaokai Yao,Gang Chang,Fuchou Tang,Xiao-Yang Zhao
View all publications

Product promise

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