AB9374

Anti-AACT antibody

Name: Anti-AACT antibody (ab9374) | Abcam
Brand: Abcam Limited
SKU: AB9374
Price: 490 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal AACT antibody. Suitable for WB, sELISA, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human SERPINA3.

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody (AB9374)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody (AB9374)

Human normal skin. Staining is mainly membrane-bound and cytoplasmic. Left panel : with primary antibody at 2 ug/ml. Right panel : negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

ELISA

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ELISA - Anti-AACT antibody (AB9374)

Standard Curve for AACT (Analyte : AACT protein (Human) (ab80517)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal to AACT (ab54693) at 5μg/ml and Detector Antibody Rabbit polyclonal to AACT (ab9374) at 0.5μg/ml.

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Western blot - Anti-AACT antibody (AB9374)

All lanes:

Western blot - Anti-AACT antibody (ab9374) at 1 µg/mL

All lanes:

Native Human AACT protein (<a href='/en-us/products/unavailable/native-human-aact-protein-ab80517'>ab80517</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 47 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P, sELISA, WB

applications

Immunogen

Native Full Length Protein corresponding to Human SERPINA3.

P01011

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Assay Dependent", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (<a href='/en-us/products/unavailable/aact-antibody-ab54693'>ab54693</a>) as Capture.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "ABC method." }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Assay Dependent", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (<a href='/en-us/products/unavailable/aact-antibody-ab54693'>ab54693</a>) as Capture.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "ABC method." }, "Mammals": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 - 7.5 Constituents: PBS, 0.01425% Magnesium chloride, 0.01% BSA, 0.00154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein Alpha-1-antichymotrypsin (AACT) also known as ACT is a serpin family member with a mass of approximately 68 kDa. This protein functions as an inhibitor of serine proteases particularly targeting chymotrypsin-like enzymes. AACT is expressed in the liver and secreted into the plasma where it carries out its inhibitory role. It is a significant component of the antiprotease shield in human physiology guarding tissues against proteolytic damage.

Biological function summary

Alpha-1-antichymotrypsin plays a role in regulating protease activity to maintain tissue integrity. AACT forms part of a complex that involves other protease inhibitors and enzymes contributing to the balance of protein breakdown and regeneration. Its presence in the extracellular matrix highlights its structural and protective functions supporting cells against enzyme-mediated degradation.

Pathways

Alpha-1-antichymotrypsin takes part in inflammatory and immune response pathways. It interacts within the acute phase response pathway a system activated during inflammatory events. This protein's relationship with other protease inhibitors such as alpha-1-antitrypsin accentuates its role in controlling proteolytic enzymes that could otherwise propagate unchecked inflammation and tissue damage.

Alpha-1-antichymotrypsin associates with neurodegenerative diseases such as Alzheimer's disease and pulmonary disorders. Its elevated levels have been observed in Alzheimer's disease cases where it might participate alongside amyloid-beta precursor protein influencing amyloid plaque formation. In lung-related disorders its collaboration with proteins like elastase can affect tissue breakdown emphasizing its role in maintaining pulmonary health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

See full target information SERPINA3

Alternative Names

AACT, GIG24, GIG25, SERPINA3, Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Clinical proteomics 14:12 PubMed28439213

2017

A clinical perspective on the utility of alpha 1 antichymotrypsin for the early diagnosis of calcific aortic stenosis.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Tatiana Martin-Rojas,Laura Mourino-Alvarez,Felix Gil-Dones,Fernando de la Cuesta,Esther Rosello-Lleti,Carlos M Laborde,Miguel Rivera,Luis Fernando Lopez-Almodovar,Juan Antonio Lopez,Finn Akerstrom,Luis R Padial,Maria G Barderas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AACT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody (AB9374)

ELISA - Anti-AACT antibody (AB9374)

Western blot - Anti-AACT antibody (AB9374)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise