Rabbit Recombinant Monoclonal AACT antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--western-blot-img54297.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)")
![Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--western-blot-img78908.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128726.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)")
![Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--immunoprecipitation-img73909.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118959.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
Alternative names
AACT, GIG24, GIG25, SERPINA3, Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3
Recommended products
Rabbit Recombinant Monoclonal AACT antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14117(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250195 is the carrier-free version of Anti-AACT antibody [EPR14117(B)] ab180492.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target protein Alpha-1-antichymotrypsin (AACT) also known as ACT is a serpin family member with a mass of approximately 68 kDa. This protein functions as an inhibitor of serine proteases particularly targeting chymotrypsin-like enzymes. AACT is expressed in the liver and secreted into the plasma where it carries out its inhibitory role. It is a significant component of the antiprotease shield in human physiology guarding tissues against proteolytic damage.
- Biological function summary
Alpha-1-antichymotrypsin plays a role in regulating protease activity to maintain tissue integrity. AACT forms part of a complex that involves other protease inhibitors and enzymes contributing to the balance of protein breakdown and regeneration. Its presence in the extracellular matrix highlights its structural and protective functions supporting cells against enzyme-mediated degradation.
- Pathways
Alpha-1-antichymotrypsin takes part in inflammatory and immune response pathways. It interacts within the acute phase response pathway a system activated during inflammatory events. This protein's relationship with other protease inhibitors such as alpha-1-antitrypsin accentuates its role in controlling proteolytic enzymes that could otherwise propagate unchecked inflammation and tissue damage.
- Associated diseases and disorders
Alpha-1-antichymotrypsin associates with neurodegenerative diseases such as Alzheimer's disease and pulmonary disorders. Its elevated levels have been observed in Alzheimer's disease cases where it might participate alongside amyloid-beta precursor protein influencing amyloid plaque formation. In lung-related disorders its collaboration with proteins like elastase can affect tissue breakdown emphasizing its role in maintaining pulmonary health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--western-blot-img54297.jpg "Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)")
Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)
This data was developed using Anti-AACT antibody [EPR14117(B)] ab180492, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-AACT antibody [EPR14117(B)] (Anti-AACT antibody [EPR14117(B)] ab180492) at 1/10000 dilution
All lanes: Human plasma lysate at 20 µg
SecondaryAll lanes: Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa
![Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--western-blot-img78908.jpg "Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)")
Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)
This data was developed using Anti-AACT antibody [EPR14117(B)] ab180492, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-AACT antibody [EPR14117(B)] (Anti-AACT antibody [EPR14117(B)] ab180492) at 1/2000 dilution
All lanes: Human colon cancer tissue lysate at 20 µg
SecondaryAll lanes: Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128726.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)
This data was developed using Anti-AACT antibody [EPR14117(B)] ab180492, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with Anti-AACT antibody [EPR14117(B)] ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--immunoprecipitation-img73909.jpg "Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)")
Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)
This data was developed using Anti-AACT antibody [EPR14117(B)] ab180492, the same antibody clone in a different buffer formulation.Western blot analysis of Human plasma immunoprecipitated using Anti-AACT antibody [EPR14117(B)] ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (Anti-AACT antibody [EPR14117(B)] ab180492)
Predicted band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195), expandable thumbnail](https://content.abcam.com/products/images/aact-antibody-epr14117b-bsa-and-azide-free-ab250195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118959.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)
This data was developed using Anti-AACT antibody [EPR14117(B)] ab180492, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with Anti-AACT antibody [EPR14117(B)] ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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