AB250195

Anti-AACT antibody [EPR14117(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal AACT antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

View Alternative Names

AACT, GIG24, GIG25, SERPINA3, Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

This data was developed using ab180492, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

This data was developed using ab180492, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

This data was developed using ab180492, the same antibody clone in a different buffer formulation.

Western blot analysis of Human plasma immunoprecipitated using ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (<a href='/en-us/products/primary-antibodies/aact-antibody-epr14117b-ab180492'>ab180492</a>)

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

This data was developed using ab180492, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AACT antibody [EPR14117(B)] (<a href='/en-us/products/primary-antibodies/aact-antibody-epr14117b-ab180492'>ab180492</a>) at 1/2000 dilution

All lanes:

Human colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 70 kDa

false

Supplier Data

Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

This data was developed using ab180492, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AACT antibody [EPR14117(B)] (<a href='/en-us/products/primary-antibodies/aact-antibody-epr14117b-ab180492'>ab180492</a>) at 1/10000 dilution

All lanes:

Human plasma lysate at 20 µg

Secondary

All lanes:

Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 70 kDa

false

Unconjugated

Anti-AACT antibody [EPR14117(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14117(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250195 is the carrier-free version of ab180492.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein Alpha-1-antichymotrypsin (AACT) also known as ACT is a serpin family member with a mass of approximately 68 kDa. This protein functions as an inhibitor of serine proteases particularly targeting chymotrypsin-like enzymes. AACT is expressed in the liver and secreted into the plasma where it carries out its inhibitory role. It is a significant component of the antiprotease shield in human physiology guarding tissues against proteolytic damage.

Biological function summary

Alpha-1-antichymotrypsin plays a role in regulating protease activity to maintain tissue integrity. AACT forms part of a complex that involves other protease inhibitors and enzymes contributing to the balance of protein breakdown and regeneration. Its presence in the extracellular matrix highlights its structural and protective functions supporting cells against enzyme-mediated degradation.

Pathways

Alpha-1-antichymotrypsin takes part in inflammatory and immune response pathways. It interacts within the acute phase response pathway a system activated during inflammatory events. This protein's relationship with other protease inhibitors such as alpha-1-antitrypsin accentuates its role in controlling proteolytic enzymes that could otherwise propagate unchecked inflammation and tissue damage.

Alpha-1-antichymotrypsin associates with neurodegenerative diseases such as Alzheimer's disease and pulmonary disorders. Its elevated levels have been observed in Alzheimer's disease cases where it might participate alongside amyloid-beta precursor protein influencing amyloid plaque formation. In lung-related disorders its collaboration with proteins like elastase can affect tissue breakdown emphasizing its role in maintaining pulmonary health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

See full target information SERPINA3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AACT antibody [EPR14117(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

Western blot - Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (AB250195)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise