AB250768

Anti-AACT antibody [EPR14118(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal AACT antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AACT, GIG24, GIG25, SERPINA3, Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3

1 Images

Supplier Data

Western blot - Anti-AACT antibody [EPR14118(B)] - BSA and Azide free (AB250768)

This data was developed using ab184567, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AACT antibody [EPR14118(B)] (<a href='/en-us/products/primary-antibodies/aact-antibody-epr14118b-ab184567'>ab184567</a>) at 1/10000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14118(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250768 is the carrier-free version of ab184567.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein Alpha-1-antichymotrypsin (AACT) also known as ACT is a serpin family member with a mass of approximately 68 kDa. This protein functions as an inhibitor of serine proteases particularly targeting chymotrypsin-like enzymes. AACT is expressed in the liver and secreted into the plasma where it carries out its inhibitory role. It is a significant component of the antiprotease shield in human physiology guarding tissues against proteolytic damage.

Biological function summary

Alpha-1-antichymotrypsin plays a role in regulating protease activity to maintain tissue integrity. AACT forms part of a complex that involves other protease inhibitors and enzymes contributing to the balance of protein breakdown and regeneration. Its presence in the extracellular matrix highlights its structural and protective functions supporting cells against enzyme-mediated degradation.

Pathways

Alpha-1-antichymotrypsin takes part in inflammatory and immune response pathways. It interacts within the acute phase response pathway a system activated during inflammatory events. This protein's relationship with other protease inhibitors such as alpha-1-antitrypsin accentuates its role in controlling proteolytic enzymes that could otherwise propagate unchecked inflammation and tissue damage.

Alpha-1-antichymotrypsin associates with neurodegenerative diseases such as Alzheimer's disease and pulmonary disorders. Its elevated levels have been observed in Alzheimer's disease cases where it might participate alongside amyloid-beta precursor protein influencing amyloid plaque formation. In lung-related disorders its collaboration with proteins like elastase can affect tissue breakdown emphasizing its role in maintaining pulmonary health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

See full target information SERPINA3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AACT antibody [EPR14118(B)] - BSA and Azide free

Western blot - Anti-AACT antibody [EPR14118(B)] - BSA and Azide free (AB250768)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise