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Rabbit Recombinant Monoclonal AACT antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-AACT antibody [EPR17088-68] (AB205198), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR17088-68] (AB205198), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody [EPR17088-68] (AB205198), expandable thumbnail
  • Western blot - Anti-AACT antibody [EPR17088-68] (AB205198), expandable thumbnail
  • Western blot - Anti-AACT antibody [EPR17088-68] (AB205198), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/40
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

Alternative names

AACT, GIG24, GIG25, SERPINA3, Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3

Recommended products

Rabbit Recombinant Monoclonal AACT antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17088-68
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The target protein Alpha-1-antichymotrypsin (AACT) also known as ACT is a serpin family member with a mass of approximately 68 kDa. This protein functions as an inhibitor of serine proteases particularly targeting chymotrypsin-like enzymes. AACT is expressed in the liver and secreted into the plasma where it carries out its inhibitory role. It is a significant component of the antiprotease shield in human physiology guarding tissues against proteolytic damage.

Biological function summary

Alpha-1-antichymotrypsin plays a role in regulating protease activity to maintain tissue integrity. AACT forms part of a complex that involves other protease inhibitors and enzymes contributing to the balance of protein breakdown and regeneration. Its presence in the extracellular matrix highlights its structural and protective functions supporting cells against enzyme-mediated degradation.

Pathways

Alpha-1-antichymotrypsin takes part in inflammatory and immune response pathways. It interacts within the acute phase response pathway a system activated during inflammatory events. This protein's relationship with other protease inhibitors such as alpha-1-antitrypsin accentuates its role in controlling proteolytic enzymes that could otherwise propagate unchecked inflammation and tissue damage.

Associated diseases and disorders

Alpha-1-antichymotrypsin associates with neurodegenerative diseases such as Alzheimer's disease and pulmonary disorders. Its elevated levels have been observed in Alzheimer's disease cases where it might participate alongside amyloid-beta precursor protein influencing amyloid plaque formation. In lung-related disorders its collaboration with proteins like elastase can affect tissue breakdown emphasizing its role in maintaining pulmonary health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Western blot - Anti-AACT antibody [EPR17088-68] (ab205198)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9880565).

    All lanes: Western blot - Anti-AACT antibody [EPR17088-68] (ab205198) at 1/2000 dilution

    All lanes: Human plasma at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 47 kDa

    Observed band size: 50-70 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR17088-68] (ab205198)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling AACT with ab205198 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab205198) at 1/500 dilution. Cytoplasmic staining on human tonsil tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody [EPR17088-68] (ab205198)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) cells labeling AACT with ab205198 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on Raji cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab205198 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Western blot - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Western blot - Anti-AACT antibody [EPR17088-68] (ab205198)

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    All lanes: Western blot - Anti-AACT antibody [EPR17088-68] (ab205198) at 1/1000 dilution

    All lanes: His-Tagged Human SERPINA3 (aa 24 to 324) recombinant protein 100ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

    Observed band size: 33 kDa

    Exposure time: 3min

  • Western blot - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Western blot - Anti-AACT antibody [EPR17088-68] (ab205198)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AACT antibody [EPR17088-68] (ab205198) at 1/1000 dilution

    All lanes: Human blood at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 47 kDa

    Observed band size: 50-70 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Immunoprecipitation - Anti-AACT antibody [EPR17088-68] (ab205198)

    AACT was immunoprecipitated from 1 mg of Human plasma lysate with ab205198 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab205198 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human plasma lysate, 10 μg (Input).

    Lane 2: ab205198 IP in Human plasma lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab205198 in Human plasma lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-AACT antibody [EPR17088-68] (ab205198)

    Predicted band size: 47 kDa

  • Western blot - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Western blot - Anti-AACT antibody [EPR17088-68] (ab205198)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AACT antibody [EPR17088-68] (ab205198) at 1/1000 dilution

    All lanes: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 47 kDa

    Observed band size: 50-70 kDa

    Exposure time: 30s

  • Western blot - Anti-AACT antibody [EPR17088-68] (ab205198), expandable thumbnail

    Western blot - Anti-AACT antibody [EPR17088-68] (ab205198)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    De-glycosylation treatment with PNGase, demonstrates a molecular weight shift from 50-70kDa to 46kDa. This result is consistent with what has been described in literature the literature (PMID:) 9880565.

    All lanes: Western blot - Anti-AACT antibody [EPR17088-68] (ab205198) at 1/2000 dilution

    Lane 1: Untreated Human plasma at 10 µg

    Lane 2: Human plasma treated with PNGase F at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 47 kDa

    Observed band size: 50-70 kDa

    Exposure time: 4s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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