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AB129194

Anti-AACT antibody [EPR5905]

1

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal AACT antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

AACT, GIG24, GIG25, SERPINA3, Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3

1 Images
Western blot - Anti-AACT antibody [EPR5905] (AB129194)
  • WB

Unknown

Western blot - Anti-AACT antibody [EPR5905] (AB129194)

All lanes:

Western blot - Anti-AACT antibody [EPR5905] (ab129194) at 1/10000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human colon cancer lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa

false

  • Carrier free

    Anti-AACT antibody [EPR5905] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5905

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein Alpha-1-antichymotrypsin (AACT) also known as ACT is a serpin family member with a mass of approximately 68 kDa. This protein functions as an inhibitor of serine proteases particularly targeting chymotrypsin-like enzymes. AACT is expressed in the liver and secreted into the plasma where it carries out its inhibitory role. It is a significant component of the antiprotease shield in human physiology guarding tissues against proteolytic damage.
Biological function summary

Alpha-1-antichymotrypsin plays a role in regulating protease activity to maintain tissue integrity. AACT forms part of a complex that involves other protease inhibitors and enzymes contributing to the balance of protein breakdown and regeneration. Its presence in the extracellular matrix highlights its structural and protective functions supporting cells against enzyme-mediated degradation.

Pathways

Alpha-1-antichymotrypsin takes part in inflammatory and immune response pathways. It interacts within the acute phase response pathway a system activated during inflammatory events. This protein's relationship with other protease inhibitors such as alpha-1-antitrypsin accentuates its role in controlling proteolytic enzymes that could otherwise propagate unchecked inflammation and tissue damage.

Alpha-1-antichymotrypsin associates with neurodegenerative diseases such as Alzheimer's disease and pulmonary disorders. Its elevated levels have been observed in Alzheimer's disease cases where it might participate alongside amyloid-beta precursor protein influencing amyloid plaque formation. In lung-related disorders its collaboration with proteins like elastase can affect tissue breakdown emphasizing its role in maintaining pulmonary health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
See full target information SERPINA3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:1185 PubMed36864027

2023

N-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Wang,Yan Ding,Ran Li,Rujun Zhang,Xuejun Ge,Ruifang Gao,Miao Wang,Yubing Huang,Fang Zhang,Bin Zhao,Wang Liao,Jie Du

Journal of sleep research 28:e12721 PubMed29961995

2018

In search of cerebrospinal fluid biomarkers of fatigue in multiple sclerosis: A proteomics study.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp O Valko,Bernd Roschitzki,Wolfgang Faigle,Jonas Grossmann,Christian Panse,Peter Biro,Micha Dambach,Donat R Spahn,Michael Weller,Roland Martin,Christian R Baumann
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com