Rabbit Polyclonal AADACL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCEH1 aa 200-350.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NCEH1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PIU2
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (PubMed:17052608). May be responsible for cholesterol ester hydrolysis in macrophages (By similarity). Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds (By similarity). May contribute to cancer pathogenesis by promoting tumor cell migration (PubMed:17052608).
Alternative names
Neutral cholesterol ester hydrolase 1, NCEH, Arylacetamide deacetylase-like 1, KIAA1363, AADACL1, NCEH1
Recommended products
Rabbit Polyclonal AADACL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCEH1 aa 200-350.
Alternative names
Neutral cholesterol ester hydrolase 1, NCEH, Arylacetamide deacetylase-like 1, KIAA1363, AADACL1, NCEH1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NCEH1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PIU2
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AADACL1 also known as Neuropathy Target Esterase is an enzyme involved in the hydrolysis of cholesterol esters and glycerolipids. It has a molecular mass of approximately 45 kDa. This enzyme localizes mainly to the cytoplasm and expresses highly in the human liver kidney and pancreas with moderate expression in brain tissues. Its role in lipid metabolism suggests importance in maintaining cellular lipid balance.
- Biological function summary
This enzyme facilitates the breakdown of certain lipid molecules making it integral in lipid homeostasis. As part of a protein complex AADACL1 interacts with other enzymes that regulate lipid metabolism playing a significant role in ensuring cellular lipid composition remains regulated. It helps in preventing the toxic accumulation of lipid substrates in cells.
- Pathways
The enzyme takes part in the neutral lipid metabolism pathway and the lysosomal storage pathway. In these pathways AADACL1 works alongside proteins like lipase and acid ceramidase which contribute to lipid degradation and turnover. Its function ensures lipid molecules get processed efficiently to maintain cellular health.
- Associated diseases and disorders
This enzyme associates with neurodegenerative diseases and metabolic disorders. Mutations or malfunctions in AADACL1 relate to hereditary neuropathy and lysosomal storage diseases. Related proteins such as glucocerebrosidase and sphingomyelinase often interplay with AADACL1 within the context of these diseases linking their functions to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-AADACL1 antibody (ab224127)
All lanes: Western blot - Anti-AADACL1 antibody (ab224127) at 0.4 µg/mL
All lanes: Human cell line CAPAN-2
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AADACL1 antibody (ab224127)
Immunohistochemical analysis of human hippocampus tissue labeling AADACL1 in the cytoplasm of neuronal cells with ab224127 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-AADACL1 antibody (ab224127)
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling AADACL1 (green) in the cytoplasm and Golgi apparatus with ab224127 at 2 μg/ml.
Downloads
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