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Rabbit Polyclonal AADACL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCEH1 aa 200-350.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human NCEH1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PIU2
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Cow
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Cow, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Cow, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Cow, Orangutan

Dilution info

-

Notes

-

Target data

Function

Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (PubMed:17052608). May be responsible for cholesterol ester hydrolysis in macrophages (By similarity). Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds (By similarity). May contribute to cancer pathogenesis by promoting tumor cell migration (PubMed:17052608).

Alternative names

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Rabbit Polyclonal AADACL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCEH1 aa 200-350.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human NCEH1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PIU2
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

This enzyme facilitates the breakdown of certain lipid molecules making it integral in lipid homeostasis. As part of a protein complex AADACL1 interacts with other enzymes that regulate lipid metabolism playing a significant role in ensuring cellular lipid composition remains regulated. It helps in preventing the toxic accumulation of lipid substrates in cells.

Activity summary

AADACL1 also known as Neuropathy Target Esterase is an enzyme involved in the hydrolysis of cholesterol esters and glycerolipids. It has a molecular mass of approximately 45 kDa. This enzyme localizes mainly to the cytoplasm and expresses highly in the human liver kidney and pancreas with moderate expression in brain tissues. Its role in lipid metabolism suggests importance in maintaining cellular lipid balance.

Pathways

The enzyme takes part in the neutral lipid metabolism pathway and the lysosomal storage pathway. In these pathways AADACL1 works alongside proteins like lipase and acid ceramidase which contribute to lipid degradation and turnover. Its function ensures lipid molecules get processed efficiently to maintain cellular health.

Associated diseases and disorders

This enzyme associates with neurodegenerative diseases and metabolic disorders. Mutations or malfunctions in AADACL1 relate to hereditary neuropathy and lysosomal storage diseases. Related proteins such as glucocerebrosidase and sphingomyelinase often interplay with AADACL1 within the context of these diseases linking their functions to disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-AADACL1 antibody (ab224127), expandable thumbnail

    Western blot - Anti-AADACL1 antibody (ab224127)

    All lanes: Western blot - Anti-AADACL1 antibody (AB224127) at 0.4 µg/mL

    All lanes: Human cell line CAPAN-2

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AADACL1 antibody (ab224127), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AADACL1 antibody (ab224127)

    Immunohistochemical analysis of human hippocampus tissue labeling AADACL1 in the cytoplasm of neuronal cells with ab224127 at 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AADACL1 antibody (ab224127), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AADACL1 antibody (ab224127)

    Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling AADACL1 (green) in the cytoplasm and Golgi apparatus with ab224127 at 2 μg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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