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AB224127

Anti-AADACL1 antibody

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(1 Publication)

Rabbit Polyclonal AADACL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCEH1 aa 200-350.

View Alternative Names

AADACL1, KIAA1363, NCEH1, Neutral cholesterol ester hydrolase 1, NCEH, Acetylalkylglycerol acetylhydrolase, Arylacetamide deacetylase-like 1, 2-acetyl MAGE hydrolase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AADACL1 antibody (AB224127)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AADACL1 antibody (AB224127)

Immunohistochemical analysis of human hippocampus tissue labeling AADACL1 in the cytoplasm of neuronal cells with ab224127 at 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-AADACL1 antibody (AB224127)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AADACL1 antibody (AB224127)

Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling AADACL1 (green) in the cytoplasm and Golgi apparatus with ab224127 at 2 μg/ml.

Western blot - Anti-AADACL1 antibody (AB224127)
  • WB

Unknown

Western blot - Anti-AADACL1 antibody (AB224127)

All lanes:

Western blot - Anti-AADACL1 antibody (ab224127) at 0.4 µg/mL

All lanes:

Human cell line CAPAN-2

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NCEH1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q6PIU2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AADACL1 also known as Neuropathy Target Esterase is an enzyme involved in the hydrolysis of cholesterol esters and glycerolipids. It has a molecular mass of approximately 45 kDa. This enzyme localizes mainly to the cytoplasm and expresses highly in the human liver kidney and pancreas with moderate expression in brain tissues. Its role in lipid metabolism suggests importance in maintaining cellular lipid balance.
Biological function summary

This enzyme facilitates the breakdown of certain lipid molecules making it integral in lipid homeostasis. As part of a protein complex AADACL1 interacts with other enzymes that regulate lipid metabolism playing a significant role in ensuring cellular lipid composition remains regulated. It helps in preventing the toxic accumulation of lipid substrates in cells.

Pathways

The enzyme takes part in the neutral lipid metabolism pathway and the lysosomal storage pathway. In these pathways AADACL1 works alongside proteins like lipase and acid ceramidase which contribute to lipid degradation and turnover. Its function ensures lipid molecules get processed efficiently to maintain cellular health.

This enzyme associates with neurodegenerative diseases and metabolic disorders. Mutations or malfunctions in AADACL1 relate to hereditary neuropathy and lysosomal storage diseases. Related proteins such as glucocerebrosidase and sphingomyelinase often interplay with AADACL1 within the context of these diseases linking their functions to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes 2-acetyl monoalkylglycerol ether (1-O-alkyl-2-acetyl-sn-glycerol), the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (PubMed : 17052608). May be responsible for the hydrolysis of cholesterol esters (such as cholesteryl (9Z-octadecenoate)) in macrophages (By similarity). Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds (By similarity). May contribute to cancer pathogenesis by promoting tumor cell migration (PubMed : 17052608).
See full target information NCEH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

microPublication biology 2025: PubMed41050330

2025

Cells stably expressing shRNA against MYO10 display altered cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna A Mas,Chase E Cristella,Vu Hao M N Phan,Lillian S Wendt,Charlotte A Rose,Abigail Ali,David F Carpio,Christine Cole,Paige Embley,Jack E Hoskins-Harris,Delia Johnson,Noelle Ledoux,Hannah W Lwin,Sarah Salah,Erin Weisbart,Stacey J Criswell,Omar Alberto Quintero-Carmona
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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