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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal AADACL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCEH1 aa 200-350.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Cow | Predicted | Predicted | Predicted |
Orangutan | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (PubMed:17052608). May be responsible for cholesterol ester hydrolysis in macrophages (By similarity). Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds (By similarity). May contribute to cancer pathogenesis by promoting tumor cell migration (PubMed:17052608).
Neutral cholesterol ester hydrolase 1, NCEH, Arylacetamide deacetylase-like 1, KIAA1363, AADACL1, NCEH1
Rabbit Polyclonal AADACL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCEH1 aa 200-350.
Neutral cholesterol ester hydrolase 1, NCEH, Arylacetamide deacetylase-like 1, KIAA1363, AADACL1, NCEH1
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This enzyme facilitates the breakdown of certain lipid molecules making it integral in lipid homeostasis. As part of a protein complex AADACL1 interacts with other enzymes that regulate lipid metabolism playing a significant role in ensuring cellular lipid composition remains regulated. It helps in preventing the toxic accumulation of lipid substrates in cells.
AADACL1 also known as Neuropathy Target Esterase is an enzyme involved in the hydrolysis of cholesterol esters and glycerolipids. It has a molecular mass of approximately 45 kDa. This enzyme localizes mainly to the cytoplasm and expresses highly in the human liver kidney and pancreas with moderate expression in brain tissues. Its role in lipid metabolism suggests importance in maintaining cellular lipid balance.
The enzyme takes part in the neutral lipid metabolism pathway and the lysosomal storage pathway. In these pathways AADACL1 works alongside proteins like lipase and acid ceramidase which contribute to lipid degradation and turnover. Its function ensures lipid molecules get processed efficiently to maintain cellular health.
This enzyme associates with neurodegenerative diseases and metabolic disorders. Mutations or malfunctions in AADACL1 relate to hereditary neuropathy and lysosomal storage diseases. Related proteins such as glucocerebrosidase and sphingomyelinase often interplay with AADACL1 within the context of these diseases linking their functions to disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-AADACL1 antibody (AB224127) at 0.4 µg/mL
All lanes: Human cell line CAPAN-2
Predicted band size: 46 kDa
Immunohistochemical analysis of human hippocampus tissue labeling AADACL1 in the cytoplasm of neuronal cells with ab224127 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling AADACL1 (green) in the cytoplasm and Golgi apparatus with ab224127 at 2 μg/ml.
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