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AB59740

Anti-AAK1 antibody

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(1 Publication)

Rabbit Polyclonal AAK1 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA1048, AAK1, AP2-associated protein kinase 1, Adaptor-associated kinase 1

1 Images
Western blot - Anti-AAK1 antibody (AB59740)
  • WB

Unknown

Western blot - Anti-AAK1 antibody (AB59740)

All lanes:

Western blot - Anti-AAK1 antibody (ab59740) at 0.5 µg/mL

Lane 1:

Mouse tissue lysate

Lane 2:

Rat kidney tissue lysate

Predicted band size: 104 kDa

Observed band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human AAK1. The exact immunogen used to generate this antibody is proprietary information.

Q2M2I8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-3 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3810 AAK1 Antibody. 3810-100 is the same size as the 100 µg size of ab59740.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AAK1 also known as adaptor-associated kinase 1 is a protein kinase with a molecular weight of 88 kDa. It plays a significant role in the regulation of clathrin-mediated endocytosis. AAK1 phosphorylates the mu2 subunit of the AP2 complex which is critical for the recruitment of clathrin to membranes. This protein is expressed in various tissues with high levels noted in the brain and spinal cord. AAK1 also participates in the modulation of neurotransmitter receptors and synaptic vesicle function.
Biological function summary

AAK1 functions as part of the AP2 clathrin adaptor complex which is essential for the internalization of cargo at the plasma membrane. Its activity is important for maintaining proper cellular uptake of nutrients receptors and signaling molecules. In neurons AAK1 regulates the cycle of synaptic vesicles impacting neurotransmission by influencing vesicle release and recycling. It has been suggested that AAK1 also contributes to the modulation of dendritic spine morphology through its kinase activity.

Pathways

AAK1 participates in endocytosis and intracellular trafficking pathways. It is directly involved in the clathrin-mediated endocytic route interacting with other proteins such as clathrin and dynamin. This association with clathrin provides a mechanism for cargo selection and membrane curvature linking AAK1 to the broader system of intracellular transport. In addition AAK1 connects to MAPK signaling through potential cross-talk mechanisms highlighting its role in cellular signaling regulation.

AAK1 has connections to neurodegeneration and cancer. In diseases like Alzheimer's abnormal function or expression of AAK1 can affect synaptic activity potentially exacerbating memory loss and cognitive decline. AAK1 also shows altered expression patterns in some cancers suggesting a role in oncogenic pathways. Its interaction with proteins like ALK and Ret may influence cell migration and proliferation reflecting AAK1's impact on disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed : 11877457, PubMed : 11877461, PubMed : 12952931, PubMed : 14617351, PubMed : 17494869, PubMed : 25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed : 17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed : 11877457, PubMed : 18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed : 12952931). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (PubMed : 18657069). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (PubMed : 21464124).. (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.
See full target information AAK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology 27:478-84 PubMed19412185

2009

Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Hu,Masayuki Matsui,Keith T Gagnon,Jacob C Schwartz,Sylvie Gabillet,Khalil Arar,Jun Wu,Ilya Bezprozvanny,David R Corey
View all publications
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