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AB248763

Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal AAK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

KIAA1048, AAK1, AP2-associated protein kinase 1, Adaptor-associated kinase 1

2 Images
Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (AB248763)
  • WB

Unknown

Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (AB248763)

This data was developed using ab134971, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AAK1 antibody [EPR5126(2)] (<a href='/en-us/products/primary-antibodies/aak1-antibody-epr51262-ab134971'>ab134971</a>) at 1/1000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 20ug

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 104 kDa

Observed band size: 140 kDa

false

Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (AB248763)
  • WB

Unknown

Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (AB248763)

This data was developed using ab134971, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AAK1 antibody [EPR5126(2)] (<a href='/en-us/products/primary-antibodies/aak1-antibody-epr51262-ab134971'>ab134971</a>) at 1/10000 dilution

All lanes:

His-tagged human AAK1 recombinant protein (aa 1-365) 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

Observed band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5126(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab248763 is the carrier-free version of ab134971.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AAK1 also known as adaptor-associated kinase 1 is a protein kinase with a molecular weight of 88 kDa. It plays a significant role in the regulation of clathrin-mediated endocytosis. AAK1 phosphorylates the mu2 subunit of the AP2 complex which is critical for the recruitment of clathrin to membranes. This protein is expressed in various tissues with high levels noted in the brain and spinal cord. AAK1 also participates in the modulation of neurotransmitter receptors and synaptic vesicle function.
Biological function summary

AAK1 functions as part of the AP2 clathrin adaptor complex which is essential for the internalization of cargo at the plasma membrane. Its activity is important for maintaining proper cellular uptake of nutrients receptors and signaling molecules. In neurons AAK1 regulates the cycle of synaptic vesicles impacting neurotransmission by influencing vesicle release and recycling. It has been suggested that AAK1 also contributes to the modulation of dendritic spine morphology through its kinase activity.

Pathways

AAK1 participates in endocytosis and intracellular trafficking pathways. It is directly involved in the clathrin-mediated endocytic route interacting with other proteins such as clathrin and dynamin. This association with clathrin provides a mechanism for cargo selection and membrane curvature linking AAK1 to the broader system of intracellular transport. In addition AAK1 connects to MAPK signaling through potential cross-talk mechanisms highlighting its role in cellular signaling regulation.

AAK1 has connections to neurodegeneration and cancer. In diseases like Alzheimer's abnormal function or expression of AAK1 can affect synaptic activity potentially exacerbating memory loss and cognitive decline. AAK1 also shows altered expression patterns in some cancers suggesting a role in oncogenic pathways. Its interaction with proteins like ALK and Ret may influence cell migration and proliferation reflecting AAK1's impact on disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed : 11877457, PubMed : 11877461, PubMed : 12952931, PubMed : 14617351, PubMed : 17494869, PubMed : 25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed : 17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed : 11877457, PubMed : 18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed : 12952931). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (PubMed : 18657069). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (PubMed : 21464124).. (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.
See full target information AAK1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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