Rabbit Polyclonal AANAT antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Mouse Aanat aa 1-50.
IgG
Rabbit
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Liquid
Polyclonal
| WB | IHC-P | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Predicted | Predicted |
| Rat | Expected | Expected |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Hamster | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Select an associated product type
Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
Serotonin N-acetyltransferase, Serotonin acetylase, Aralkylamine N-acetyltransferase, AA-NAT, Aanat, Snat
Rabbit Polyclonal AANAT antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Mouse Aanat aa 1-50.
IgG
Rabbit
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
AANAT also known as aralkylamine N-acetyltransferase or serotonin N-acetyltransferase is an enzyme with a molecular mass of approximately 23.3 kDa. This enzyme catalyzes the transfer of an acetyl group from acetyl-CoA to serotonin forming N-acetylserotonin. AANAT expression is mainly found in the pineal gland where it acts as an important player in melatonin biosynthesis. The function of AANAT as a regulatory point in this biosynthesis contributes to the role of melatonin in circadian rhythms and sleep regulation.
AANAT has a critical role in regulating physiological processes through the production of melatonin. It operates as part of the intramolecular complex that facilitates its enzymatic activity in the pineal gland. By converting serotonin into N-acetylserotonin which further converts to melatonin AANAT modulates the synthesis of melatonin an indoleamine with effects on sleep-wake cycles and seasonal reproductive rhythms in mammals. This regulatory activity reflects how AANAT influences the hormonal signaling pathways in response to light and dark cycles.
AANAT is integral to the melatonin synthesis pathway and interacts closely in serotonin metabolism. It tightly regulates the conversion of serotonin to melatonin in the pineal gland especially during nocturnal periods. One key protein related to AANAT in this pathway is hydroxyindole O-methyltransferase (HIOMT) which converts N-acetylserotonin to melatonin. The modulation of melatonin levels impacts diverse biological pathways including circadian rhythm regulation and neuroendocrine signaling.
AANAT plays a significant role in conditions like seasonal affective disorder (SAD) and sleep disturbances. Alterations in AANAT expression or function can affect melatonin levels leading to these disorders. Additionally disruptions in the enzymatic activity of AANAT may have interactions with serotonin influencing its availability and potentially linking it to disorders like depression. Understanding AANAT's function allows researchers to explore therapeutic strategies targeting melatonin pathways in managing these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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