AB3505

Anti-AANAT antibody

Name: Anti-AANAT antibody (ab3505) | Abcam
Brand: Abcam Limited
SKU: AB3505
Price: 535 USD
Availability: InStock

(1 Review)

(20 Publications)

Rabbit Polyclonal AANAT antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Mouse Aanat aa 1-50.

View Alternative Names

Snat, Aanat, Serotonin N-acetyltransferase, Serotonin acetylase, Aralkylamine N-acetyltransferase, AA-NAT

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Aanat aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O88816

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AANAT also known as aralkylamine N-acetyltransferase or serotonin N-acetyltransferase is an enzyme with a molecular mass of approximately 23.3 kDa. This enzyme catalyzes the transfer of an acetyl group from acetyl-CoA to serotonin forming N-acetylserotonin. AANAT expression is mainly found in the pineal gland where it acts as an important player in melatonin biosynthesis. The function of AANAT as a regulatory point in this biosynthesis contributes to the role of melatonin in circadian rhythms and sleep regulation.

Biological function summary

AANAT has a critical role in regulating physiological processes through the production of melatonin. It operates as part of the intramolecular complex that facilitates its enzymatic activity in the pineal gland. By converting serotonin into N-acetylserotonin which further converts to melatonin AANAT modulates the synthesis of melatonin an indoleamine with effects on sleep-wake cycles and seasonal reproductive rhythms in mammals. This regulatory activity reflects how AANAT influences the hormonal signaling pathways in response to light and dark cycles.

Pathways

AANAT is integral to the melatonin synthesis pathway and interacts closely in serotonin metabolism. It tightly regulates the conversion of serotonin to melatonin in the pineal gland especially during nocturnal periods. One key protein related to AANAT in this pathway is hydroxyindole O-methyltransferase (HIOMT) which converts N-acetylserotonin to melatonin. The modulation of melatonin levels impacts diverse biological pathways including circadian rhythm regulation and neuroendocrine signaling.

AANAT plays a significant role in conditions like seasonal affective disorder (SAD) and sleep disturbances. Alterations in AANAT expression or function can affect melatonin levels leading to these disorders. Additionally disruptions in the enzymatic activity of AANAT may have interactions with serotonin influencing its availability and potentially linking it to disorders like depression. Understanding AANAT's function allows researchers to explore therapeutic strategies targeting melatonin pathways in managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

See full target information Aanat

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 13: PubMed39765828

2025

Protective Effects of Exogenous Melatonin Administration on White Fat Metabolism Disruption Induced by Aging and a High-Fat Diet in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dongying Lv,Yujie Ren,Jiayan Chen,Ziyao Pang,Yaxuan Tang,Lizong Zhang,Laiqing Yan,Xiufeng Ai,Xiaoping Xv,Dejun Wang,Zhaowei Cai

Reproduction, fertility, and development 35:563-574 PubMed37290449

2023

The melatonin system is expressed in the ovine uterus: effect of the day of the oestrous cycle and undernutrition.

Applications

Unspecified application

Species

Unspecified reactive species

C Sosa,E Laurenzana,V de Brun,A Meikle,J A Abecia

Journal of pineal research 75:e12885 PubMed37183291

2023

RP58 knockdown contributes to hypoxia-ischemia-induced pineal dysfunction and circadian rhythm disruption in neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Zubin Zhang,Xin Ding,Xiaolu Jiang,Lanlan Tan,Chenjie Lin,Lixiao Xu,Gen Li,Lianghua Lu,Zhenghong Qin,Xing Feng,Mei Li

International journal of molecular sciences 24: PubMed37108290

2023

The Uterine Melatonergic Systems of AANAT and Melatonin Membrane Receptor 2 (MT2) Are Essential for Endometrial Receptivity and Early Implantation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ma,Jing Wang,Likai Wang,Laiqing Yan,Yunjie Liu,Wenkui Ma,Pengyun Ji,Lu Zhang,Guoshi Liu

Antioxidants (Basel, Switzerland) 12: PubMed36978937

2023

Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Likai Wang,Laiqing Yan,Qi Han,Guangdong Li,Hao Wu,Xiao Ma,Mengmeng Zhao,Wenkui Ma,Pengyun Ji,Ran Zhang,Guoshi Liu

International journal of molecular sciences 23: PubMed36555116

2022

Circ-ERC2 Is Involved in Melatonin Synthesis by Regulating the miR-125a-5p/MAT2A Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Xiang Guo,Yi Zheng,Guo-Kun Zhao,Hao-Qi Wang,Song Yu,Fei Gao,Jia-Bao Zhang,Yong-Hong Zhang,Bao Yuan

Cellular and molecular gastroenterology and hepatology 14:877-904 PubMed35863741

2022

Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ludovica Ceci,Lixian Chen,Leonardo Baiocchi,Nan Wu,Lindsey Kennedy,Guido Carpino,Konstantina Kyritsi,Tianhao Zhou,Travis Owen,Debjyoti Kundu,Amelia Sybenga,Abdulkadir Isidan,Burcin Ekser,Antonio Franchitto,Paolo Onori,Eugenio Gaudio,Romina Mancinelli,Heather Francis,Gianfranco Alpini,Shannon Glaser

Nutrients 14: PubMed35010992

2021

Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lijin Song,Meibo He,Qinghua Sun,Yujing Wang,Jindong Zhang,Yuan Fang,Shuangjiang Liu,Liping Duan

Communications biology 4:534 PubMed33958705

2021

Melatonin delays ovarian aging in mice by slowing down the exhaustion of ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Yang,Qinghua Liu,Yingjun Chen,Xiaodong Wang,Zaohong Ran,Fang Fang,Jiajun Xiong,Guoshi Liu,Xiang Li,Liguo Yang,Changjiu He

Frontiers in cell and developmental biology 9:648148 PubMed33937242

2021

Melatonin Alleviates the Suppressive Effect of Hypoxanthine on Oocyte Nuclear Maturation and Restores Meiosis via the Melatonin Receptor 1 (MT1)-Mediated Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Zhiyong Zhuo,Xiao Ma,Yunjie Liu,Jing Xu,Changjiu He,Yao Fu,Feng Wang,Pengyun Ji,Lu Zhang,Guoshi Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AANAT antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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