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AB3506

Anti-AANAT antibody

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(2 Publications)

Rabbit Polyclonal AANAT antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Aanat aa 150 to C-terminus.

View Alternative Names

SNAT, AANAT, Serotonin N-acetyltransferase, Serotonin acetylase, Aralkylamine N-acetyltransferase, AA-NAT

1 Images
Western blot - Anti-AANAT antibody (AB3506)
  • WB

Unknown

Western blot - Anti-AANAT antibody (AB3506)

Western blot of Serotonin N-acetyltransferase in transfected 293T cell lysate using ab3506.

All lanes:

Western blot - Anti-AANAT antibody (ab3506)

Predicted band size: 23 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Rat Aanat aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q64666

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Total anti-C-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific carboxy-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AANAT also known as aralkylamine N-acetyltransferase or serotonin N-acetyltransferase is an enzyme with a molecular mass of approximately 23.3 kDa. This enzyme catalyzes the transfer of an acetyl group from acetyl-CoA to serotonin forming N-acetylserotonin. AANAT expression is mainly found in the pineal gland where it acts as an important player in melatonin biosynthesis. The function of AANAT as a regulatory point in this biosynthesis contributes to the role of melatonin in circadian rhythms and sleep regulation.
Biological function summary

AANAT has a critical role in regulating physiological processes through the production of melatonin. It operates as part of the intramolecular complex that facilitates its enzymatic activity in the pineal gland. By converting serotonin into N-acetylserotonin which further converts to melatonin AANAT modulates the synthesis of melatonin an indoleamine with effects on sleep-wake cycles and seasonal reproductive rhythms in mammals. This regulatory activity reflects how AANAT influences the hormonal signaling pathways in response to light and dark cycles.

Pathways

AANAT is integral to the melatonin synthesis pathway and interacts closely in serotonin metabolism. It tightly regulates the conversion of serotonin to melatonin in the pineal gland especially during nocturnal periods. One key protein related to AANAT in this pathway is hydroxyindole O-methyltransferase (HIOMT) which converts N-acetylserotonin to melatonin. The modulation of melatonin levels impacts diverse biological pathways including circadian rhythm regulation and neuroendocrine signaling.

AANAT plays a significant role in conditions like seasonal affective disorder (SAD) and sleep disturbances. Alterations in AANAT expression or function can affect melatonin levels leading to these disorders. Additionally disruptions in the enzymatic activity of AANAT may have interactions with serotonin influencing its availability and potentially linking it to disorders like depression. Understanding AANAT's function allows researchers to explore therapeutic strategies targeting melatonin pathways in managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
See full target information AANAT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e55681 PubMed36744362

2023

FTO negatively regulates the cytotoxic activity of natural killer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seok-Min Kim,Se-Chan Oh,Sun-Young Lee,Ling-Zu Kong,Jong-Hee Lee,Tae-Don Kim

The Journal of allergy and clinical immunology 141:1382-1394.e14 PubMed28797734

2017

MicroRNA-150 controls differentiation of intraepithelial lymphocytes through TGF-β receptor II regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Hwan Seo,Min Seong Jang,Doo-Jin Kim,Seok-Min Kim,Se-Chan Oh,Cho-Rok Jung,Yunji Park,Sang-Jun Ha,Haiyoung Jung,Young-Jun Park,Suk Ran Yoon,Inpyo Choi,Tae-Don Kim
View all publications
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