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AB3439

Anti-AANAT (phospho T29) antibody

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(4 Publications)

Rabbit Polyclonal AANAT phospho T29 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Mouse Aanat phospho T29 aa 1-50.

View Alternative Names

Snat, Serotonin N-acetyltransferase, Serotonin acetylase, Aralkylamine N-acetyltransferase, AA-NAT

1 Images
Western blot - Anti-AANAT (phospho T29) antibody (AB3439)
  • WB

Unknown

Western blot - Anti-AANAT (phospho T29) antibody (AB3439)

Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.

All lanes:

Western blot - Anti-AANAT (phospho T29) antibody (ab3439)

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Mouse Aanat phospho T29 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O88816

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p>By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide.Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AANAT also known as aralkylamine N-acetyltransferase or serotonin N-acetyltransferase is an enzyme with a molecular mass of approximately 23.3 kDa. This enzyme catalyzes the transfer of an acetyl group from acetyl-CoA to serotonin forming N-acetylserotonin. AANAT expression is mainly found in the pineal gland where it acts as an important player in melatonin biosynthesis. The function of AANAT as a regulatory point in this biosynthesis contributes to the role of melatonin in circadian rhythms and sleep regulation.
Biological function summary

AANAT has a critical role in regulating physiological processes through the production of melatonin. It operates as part of the intramolecular complex that facilitates its enzymatic activity in the pineal gland. By converting serotonin into N-acetylserotonin which further converts to melatonin AANAT modulates the synthesis of melatonin an indoleamine with effects on sleep-wake cycles and seasonal reproductive rhythms in mammals. This regulatory activity reflects how AANAT influences the hormonal signaling pathways in response to light and dark cycles.

Pathways

AANAT is integral to the melatonin synthesis pathway and interacts closely in serotonin metabolism. It tightly regulates the conversion of serotonin to melatonin in the pineal gland especially during nocturnal periods. One key protein related to AANAT in this pathway is hydroxyindole O-methyltransferase (HIOMT) which converts N-acetylserotonin to melatonin. The modulation of melatonin levels impacts diverse biological pathways including circadian rhythm regulation and neuroendocrine signaling.

AANAT plays a significant role in conditions like seasonal affective disorder (SAD) and sleep disturbances. Alterations in AANAT expression or function can affect melatonin levels leading to these disorders. Additionally disruptions in the enzymatic activity of AANAT may have interactions with serotonin influencing its availability and potentially linking it to disorders like depression. Understanding AANAT's function allows researchers to explore therapeutic strategies targeting melatonin pathways in managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
See full target information Aanat phospho T29
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Journal of pineal research 75:e12909 PubMed37721126

2023

Biosynthesis of neuroprotective melatonin is dysregulated in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jinho Kim,Wei Li,Jingjing Wang,Sergei V Baranov,Brianna E Heath,Jiaoying Jia,Yalikun Suofu,Oxana V Baranova,Xiaomin Wang,Timothy M Larkin,William R Lariviere,Diane L Carlisle,Robert M Friedlander

Frontiers in cellular neuroscience 11:98 PubMed28473754

2017

Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats.

Applications

IHC (PFA fixed)

Species

Rat

Baoke Hou,Yan Fu,Chuanhuang Weng,Weiping Liu,Congjian Zhao,Zheng Qin Yin

The British journal of nutrition 104:637-46 PubMed20338074

2010

Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew W Moran,Miran A Al-Rammahi,Daleep K Arora,Daniel J Batchelor,Erin A Coulter,Kristian Daly,Catherine Ionescu,David Bravo,Soraya P Shirazi-Beechey
View all publications
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Product promise

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