AB154800

Anti-AASS antibody [EPR9145(B)]

RabMAb
Recombinant
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(2 Publications )

Rabbit Recombinant Monoclonal AASS antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

1 Images

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Western blot - Anti-AASS antibody [EPR9145(B)] (AB154800)

All lanes:

Western blot - Anti-AASS antibody [EPR9145(B)] (ab154800) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

HUVEC lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Lane 5:

293T lysate at 10 µg

Predicted band size: 102 kDa

false

Carrier free

Anti-AASS antibody [EPR9145(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9145(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AASS also known as Aminoadipate-semialdehyde Synthase is a mitochondrial enzyme that plays a role in lysine degradation. It has alternative names like LKRSDH or Lysine Ketoglutarate Reductase/Saccharopine Dehydrogenase. AASS is a bifunctional enzyme that catalyzes two sequential reactions in lysine catabolism. The first reaction converts lysine into saccharopine and the second converts saccharopine to aminoadipate semialdehyde. The protein possesses an approximate mass of 125 kDa. The expression of AASS occurs mainly in liver and kidney tissues although it can be detected at lower levels in other organs.

Biological function summary

Enzymes like AASS are critical in the metabolism of amino acids particularly within the lysine degradation pathway. The AASS enzyme does not form a larger complex but its function is essential for preventing lysine accumulation in cells. When lysine gets degraded it assists in maintaining cellular homeostasis by producing metabolites necessary for energy production and other metabolic processes. AASS functions independently without forming catalytic subunits with other proteins.

Pathways

Lysine degradation involves AASS as a significant player. This pathway leads to the production of acetyl-CoA an essential molecule in the citric acid cycle and energy production. Another important pathway involving AASS is the production of glutaryl-CoA. During these pathways AASS-related proteins include other enzymes like saccharopine reductase and dehydrogenase which participate sequentially to manage amino acid breakdown.

Abnormal AASS function is associated with hyperlysinemia and saccharopinuria. Hyperlysinemia results from disrupted lysine degradation leading to excess lysine in the blood. Researchers have noted that AASS mutations may impact lysine metabolism causing these conditions. In comparison saccharopinuria characterized by excess saccharopine in urine directly connects to inadequate AASS activity. These conditions highlight how changes in AASS function can cause metabolic imbalances leading to disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bifunctional enzyme that catalyzes the first two steps in lysine degradation.

See full target information Alpha-aminoadipic semialdehyde synthase, mitochondrial

Alternative Names

LKR/SDH, AASS

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:22569 PubMed36581661

2022

Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Isono,Takehiro Matsumoto,Masafumi Suzaki,Shigehisa Kubota,Susumu Kageyama,Akihiro Kawauchi,Atuyuki Wada

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:2631-2646 PubMed35135854

2022

The Metabolite Saccharopine Impairs Neuronal Development by Inhibiting the Neurotrophic Function of Glucose-6-Phosphate Isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Guo,Junjie Wu,Min Wang,Xin Wang,Youli Jian,Chonglin Yang,Weixiang Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AASS antibody [EPR9145(B)]

Western blot - Anti-AASS antibody [EPR9145(B)] (AB154800)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (2)

Product promise