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AB219073

Anti-AATF/DED antibody [3C7]

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(1 Publication)

Mouse Monoclonal AATF/DED antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AATF aa 150-250.

View Alternative Names

CHE1, DED, HSPC277, AATF, Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AATF/DED antibody [3C7] (AB219073)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AATF/DED antibody [3C7] (AB219073)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling AATF/DED using ab219073 at 10 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [3C7] (AB219073)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [3C7] (AB219073)

Immunofluorescent analysis of HeLa cells labeling AATF/DED using ab219073 at 10 μg/mL.

Western blot - Anti-AATF/DED antibody [3C7] (AB219073)
  • WB

Supplier Data

Western blot - Anti-AATF/DED antibody [3C7] (AB219073)

All lanes:

Western blot - Anti-AATF/DED antibody [3C7] (ab219073)

All lanes:

HeLa cell lysate

Predicted band size: 63 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3C7

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AATF aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NY61

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AATF also known as apoptosis antagonizing transcription factor and sometimes referred to as DED a.k.a. death effector detach protein is a pivotal regulator of cellular processes. This protein has a molecular mass of approximately 61 kDa. AATF is expressed in various tissues including the brain and liver where it plays a diverse role in cellular function. As an antagonist of apoptosis AATF interacts with signaling pathways to modulate cell survival and proliferation.
Biological function summary

The protein is involved in numerous cellular activities such as transcriptional regulation and cell cycle control. AATF associates with nucleoprotein complexes and is critical in regulating transcription by interacting with RNA polymerase II. It acts as a transcriptional co-regulator influencing genes responsible for cell growth and survival. Research indicates that AATF can affect the balance between cell survival and death which is significant in both development and response to cellular stress.

Pathways

AATF interacts with multiple signaling cascades notably the apoptosis and cell cycle pathways. For example AATF participates in the p53 pathway influencing cellular responses to DNA damage. It interacts with other proteins such as p21 and cyclin D1 helping to modulate cell cycle progression and ensuring genomic stability. These interactions highlight AATF's role in maintaining cellular homeostasis and regulating programmed cell death processes.

AATF is associated with cancer and neurodegenerative diseases. Its ability to inhibit apoptosis and regulate cell proliferation links it to tumorigenesis. In cancer AATF's interaction with proteins like Bcl-2 can prevent the initiation of apoptosis contributing to tumor cell survival. Additionally imbalance in AATF activity may influence the progression of neurodegenerative conditions where its regulatory role on apoptosis and cell cycle can affect neuronal survival and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
See full target information AATF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of general physiology 157: PubMed40763259

2025

GRIN2B disease-associated mutations disrupt the function of BK channels and NMDA receptor signaling nanodomains.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Martínez-Lázaro,Teresa Minguez-Viñas,Andrea Reyes-Carrión,Ricardo Gómez,Diego Alvarez de la Rosa,David Bartolomé-Martín,Teresa Giraldez
View all publications
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Product promise

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