Rabbit Polyclonal AATF/DED antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AATF aa 500 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human AATF aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY61
Reactivity data
Select an application| IP | |
|---|---|
| Human | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
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Target data
Function
Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Alternative names
CHE1, DED, HSPC277, AATF, Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1
Recommended products
Rabbit Polyclonal AATF/DED antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AATF aa 500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human AATF aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY61
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AATF also known as apoptosis antagonizing transcription factor and sometimes referred to as DED a.k.a. death effector detach protein is a pivotal regulator of cellular processes. This protein has a molecular mass of approximately 61 kDa. AATF is expressed in various tissues including the brain and liver where it plays a diverse role in cellular function. As an antagonist of apoptosis AATF interacts with signaling pathways to modulate cell survival and proliferation.
- Biological function summary
The protein is involved in numerous cellular activities such as transcriptional regulation and cell cycle control. AATF associates with nucleoprotein complexes and is critical in regulating transcription by interacting with RNA polymerase II. It acts as a transcriptional co-regulator influencing genes responsible for cell growth and survival. Research indicates that AATF can affect the balance between cell survival and death which is significant in both development and response to cellular stress.
- Pathways
AATF interacts with multiple signaling cascades notably the apoptosis and cell cycle pathways. For example AATF participates in the p53 pathway influencing cellular responses to DNA damage. It interacts with other proteins such as p21 and cyclin D1 helping to modulate cell cycle progression and ensuring genomic stability. These interactions highlight AATF's role in maintaining cellular homeostasis and regulating programmed cell death processes.
- Associated diseases and disorders
AATF is associated with cancer and neurodegenerative diseases. Its ability to inhibit apoptosis and regulate cell proliferation links it to tumorigenesis. In cancer AATF's interaction with proteins like Bcl-2 can prevent the initiation of apoptosis contributing to tumor cell survival. Additionally imbalance in AATF activity may influence the progression of neurodegenerative conditions where its regulatory role on apoptosis and cell cycle can affect neuronal survival and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunoprecipitation - Anti-AATF/DED antibody (ab264167)
AATF/DED was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264167 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using a different AATF/DED antibody at 1 μg/ml.
Lane 1: ab264167 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 10 secs.
All lanes: Immunoprecipitation - Anti-AATF/DED antibody (ab264167)
Predicted band size: 63 kDa
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