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AB233546

Anti-AATF/DED antibody [EPR22934-136]

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(1 Publication)

Rabbit Recombinant Monoclonal AATF/DED antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

CHE1, DED, HSPC277, AATF, Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (AB233546)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (AB233546)

Immunofluorescent analysis of 4% paraformaldehyde -fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling AATF/DED with ab233546 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining in HEK-293T cells. The nuclear counte stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunoprecipitation - Anti-AATF/DED antibody [EPR22934-136] (AB233546)
  • IP

Unknown

Immunoprecipitation - Anti-AATF/DED antibody [EPR22934-136] (AB233546)

AATF/DED was immunoprecipitated from 0.35 mgof HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab233546 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233546 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : HEK-293T whole cell lysate 10 μg (Input).
Lane 2 : ab233546 IP in HEK-293T whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab233546 in HEK-293T whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-AATF/DED antibody [EPR22934-136] (ab233546)

Predicted band size: 63 kDa

Observed band size: 80 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (AB233546)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (AB233546)

Immunofluorescent analysis of 4% paraformaldehyde -fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling AATF/DED with ab233546 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining in NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Western blot - Anti-AATF/DED antibody [EPR22934-136] (AB233546)
  • WB

Unknown

Western blot - Anti-AATF/DED antibody [EPR22934-136] (AB233546)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19911006, 22933572).

All lanes:

Western blot - Anti-AATF/DED antibody [EPR22934-136] (ab233546) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 63 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

Western blot - Anti-AATF/DED antibody [EPR22934-136] (AB233546)
  • WB

Unknown

Western blot - Anti-AATF/DED antibody [EPR22934-136] (AB233546)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19911006, 22933572).

All lanes:

Western blot - Anti-AATF/DED antibody [EPR22934-136] (ab233546) at 1/1000 dilution

Lane 1:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 63 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-AATF/DED antibody [EPR22934-136] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22934-136

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AATF also known as apoptosis antagonizing transcription factor and sometimes referred to as DED a.k.a. death effector detach protein is a pivotal regulator of cellular processes. This protein has a molecular mass of approximately 61 kDa. AATF is expressed in various tissues including the brain and liver where it plays a diverse role in cellular function. As an antagonist of apoptosis AATF interacts with signaling pathways to modulate cell survival and proliferation.
Biological function summary

The protein is involved in numerous cellular activities such as transcriptional regulation and cell cycle control. AATF associates with nucleoprotein complexes and is critical in regulating transcription by interacting with RNA polymerase II. It acts as a transcriptional co-regulator influencing genes responsible for cell growth and survival. Research indicates that AATF can affect the balance between cell survival and death which is significant in both development and response to cellular stress.

Pathways

AATF interacts with multiple signaling cascades notably the apoptosis and cell cycle pathways. For example AATF participates in the p53 pathway influencing cellular responses to DNA damage. It interacts with other proteins such as p21 and cyclin D1 helping to modulate cell cycle progression and ensuring genomic stability. These interactions highlight AATF's role in maintaining cellular homeostasis and regulating programmed cell death processes.

AATF is associated with cancer and neurodegenerative diseases. Its ability to inhibit apoptosis and regulate cell proliferation links it to tumorigenesis. In cancer AATF's interaction with proteins like Bcl-2 can prevent the initiation of apoptosis contributing to tumor cell survival. Additionally imbalance in AATF activity may influence the progression of neurodegenerative conditions where its regulatory role on apoptosis and cell cycle can affect neuronal survival and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
See full target information AATF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:582 PubMed40619436

2025

IFNα-IFITM1-ERK axis in corneal epithelial cell damage: implications for dry eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjuan Peng,Siyi Li,Guiling Zhao,Suyin Peng,Yanhua Pang
View all publications
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Product promise

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