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AB324855

Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free

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Mouse Recombinant Monoclonal AAV2 antibody. Carrier free. Suitable for Dot, I-ELISA and reacts with Recombinant fragment - Adeno-associated virus 2, Recombinant fragment - Adeno-associated virus 3B samples.
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Indirect ELISA - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)

This data was developed using ab322961, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322961 at 1000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ Fragment Specific at 1 : 2000 dilution dilution.

Antigen : AAV1 empty capsid, AAV2 empty capsid, AAV3B empty capsid, AAV5 empty capsid, AAV6 empty capsid, AAV8 empty capsid, AAV9 empty capsid.

Antigen concentration : 1.0E+10 VP/ml

Dot Blot - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)
  • Dot

Supplier Data

Dot Blot - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)

This data was developed using ab322961, the same antibody clone in a different buffer formulation.

Dot blot analysis of AAV2 + AAV3B using ab322961 at 1 : 1000 (1.05 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 2000 dilution.

Lane1 : AAV1
Lane2 : AAV2
Lane3 : AAV3B
Lane4 : AAV5
Lane5 : AAV6
Lane6 : AAV8
Lane7 : AAV9

Exposure time : 59 seconds.

Blocking and diluting buffer and concentration : 2% BSA.

Anti-AAV2 antibody [EPR27877] (ab322961) (1 : 1000) recognizes AAV2/3B capsid.

All lanes:

Dot Blot - Anti-AAV2 + AAV3B antibody [EPR27877] (<a href='/en-us/products/primary-antibodies/aav2-aav3b-antibody-epr27877-ab322961'>ab322961</a>) at 1/1000 dilution

Lane 1:

AAV1 at 10 ng

Lane 2:

AAV2 at 10 ng

Lane 3:

AAV3B at 10 ng

Lane 4:

AAV5 at 10 ng

Lane 5:

AAV6 at 10 ng

Lane 6:

AAB8 at 10 ng

Lane 7:

AAV9 at 10 ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/2000 dilution

false

Exposure time: 59s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EPR27877

Isotype

IgG3

Carrier free

Yes

Reacts with

Adeno-associated virus 2, Adeno-associated virus 3B

Applications

Dot, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has the same CDR sequence as clone A20 as described in McCraw etal. Structure of adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20. Virology 431: 40-49.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Adeno-associated virus 2": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Adeno-associated virus 3B": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Adeno-associated virus 2": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Recombinant fragment - Adeno-associated virus 3B": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - Adeno-associated virus 2": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information AAV2

Additional targets

AAV3B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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