Rabbit Recombinant Monoclonal CAPSD antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra), IP and reacts with Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982), Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | ICC/IF | WB | Flow Cyt (Intra) | IP | |
---|---|---|---|---|---|
Adeno-associated virus 2 (isolate Srivastava/1982) | Predicted | Predicted | Predicted | Predicted | Predicted |
Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982) | Tested | Tested | Not recommended | Tested | Not recommended |
Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) | Not recommended | Not recommended | Tested | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982) | Dilution info - | Notes - |
Select an associated product type
Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.
Capsid protein VP1, VP1
Rabbit Recombinant Monoclonal CAPSD antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra), IP and reacts with Transfected cell line - Adeno-associated virus 2 (isolate Srivastava/1982), Transfected cell lysate - Adeno-associated virus 2 (isolate Srivastava/1982) samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR28595-59
Affinity purification Protein A
This antibody cross-react with VP1 from AAV1, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8 and AAV9 strains.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The AAV2 Capsid protein VP1 is a structural component of the adeno-associated virus serotype 2 (AAV2) capsid. It is sometimes called AAV2 VP1 and is one of the three viral proteins with a molecular mass of approximately 87 kDa. This protein is synthesized in host cells during viral replication and is expressed as part of the viral capsid the protein shell that encases and protects the viral genome. The capsid forms through the assembly of 60 viral proteins including VP1 each contributing to the structural integrity necessary for infectivity.
AAV2 Capsid protein VP1 plays an important role in viral infectivity by mediating the virus's ability to enter host cells. The capsid consisting of VP1 VP2 and VP3 proteins facilitates binding to the receptor on the host cell surface initiating the infection process. VP1 specifically contains phospholipase A2 activity which becomes essential during the entry and uncoating of the virus once inside the host cell. This functional aspect of VP1 is vital for the successful translocation of the viral DNA into the host nucleus.
The AAV2 Capsid protein VP1 involves itself in the endocytosis pathway as the virus enters the host cell. It is important in the trafficking process from the endosome to the nucleus. During this journey VP1 interacts with other viral proteins including VP2 and VP3 to form the complete capsid. Furthermore it might partake in cellular stress response pathways due to its influence on host cell structures although this warrants more exploration.
AAV2 Capsid protein VP1 finds relevance in gene therapy approaches. AAV2 vectors incorporating VP1 are employed to deliver genetic material for therapeutic purposes addressing conditions like hemophilia and cystic fibrosis. The protein's interaction with target cells and its capacity to mediate gene delivery make it central in these therapeutic strategies. VP1 together with VP2 and VP3 forms an integral part of the recombinant AAV used in these treatments exemplifying its importance in emerging medical therapies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-AAV2 Capsid protein VP1 antibody [EPR28595-59] (ab315287) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a myc-His-tag®, whole cell lysate at 5 µg
Lane 2: 293T cells transfected with a AAV-2 VP1 expression vector containi a myc-His-tag®, whole cell lysate at 5 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 81 kDa, 36 kDa
Exposure time: 10s
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling AAV2 Capsid protein VP1 with ab315287 at 1/5000 (0.106 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on rat cerebrum.
The section was incubated with ab315287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling AAV2 Capsid protein VP1 with ab315287 at 1/5000 (0.106 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on mouse cerebrum.
The section was incubated with ab315287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling AAV2 Capsid protein VP1 with ab315287 at 1/5000 (0.106 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on human cerebrum.
The section was incubated with ab315287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded (A)HEK-293T (human embryonic kidney epithelial cell) transfected with a VVA2-VP1 expression vector containing a myc tag. (B) HEK-293T transfected with an empty vector. tissue labeling AAV2 Capsid protein VP1 with ab315287 at 1/5000 (0.106 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a VVA2-VP1 expression vector containing a myc tag, no staining on (B) HEK-293T transfected with an empty vector.
The section was incubated with ab315287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with a VVA2-VP1 expression vector containing a myc tag cells labelling AAV2 Capsid protein VP1 with ab315287 at 1/50 (10.6 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing nuclear staining in 293T transfected with a VVA2-VP1 expression vector containing a myc tag.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T transfected with a VVA2-VP1 expression vector containing a myc tag (Middle) / 293T transfected with an empty expression vector containing a myc tag (Right) cells labelling AAV2 Capsid protein VP1 with ab315287 at 1/50 dilution (1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
AAV2 Capsid protein VP1 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a AAV-2 VP1 expression vector containing a myc-His-tag®, whole cell lysate with ab315287 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315287 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a AAV-2 VP1 expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: ab315287 IP in 293T (human embryonic kidney epithelial cell) cells transfected with a AAV-2 VP1 expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315287 in 293T cells transfected with a AAV-2 VP1 expression vector containing a myc-His-tag®, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-AAV2 Capsid protein VP1 antibody [EPR28595-59] (ab315287) at 1/30 dilution
All lanes: 293T (human embryonic kidney epithelial cell) cells transfected with a AAV-2 VP1 expression vector containing a myc-His-tag®, whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
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