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AB315831

Anti-AAV5 antibody [HL2476]

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Mouse Recombinant Monoclonal AAV5 antibody. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - adeno-associated virus 5 samples.
2 Images
Indirect ELISA - Anti-AAV5 antibody [HL2476] (AB315831)
  • I-ELISA

Lab

Indirect ELISA - Anti-AAV5 antibody [HL2476] (AB315831)

Indirect ELISA showing ab315831 binding to AAV5 empty capsid. Binding of ab315831 was assessed in a serial dilution and EC-50 is 1.974 ng/ml. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.

Antigen : AAV1 empty capsid,AAV2 empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid AAV3B empty capsid.

Antigen concentration : 7.5E+10 vp/mL

Dot Blot - Anti-AAV5 antibody [HL2476] (AB315831)
  • Dot

Supplier Data

Dot Blot - Anti-AAV5 antibody [HL2476] (AB315831)

Dot blot analysis of AAV5 using ab315831 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

false

Exposure time: 8min

  • Carrier free

    Anti-AAV5 antibody [HL2476] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HL2476

Isotype

IgG2b

Carrier free

No

Reacts with

adeno-associated virus 5

Applications

Dot, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - adeno-associated virus 5": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/2000", "Dot-species-notes": "<p></p>" }, "adeno-associated virus 5": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AAV5 or Adeno-Associated Virus type 5 is a non-pathogenic virus used widely as a vector in gene therapy. This virus has a protein structure named the AAV5 capsid which serves as its protective shell. The AAV5 capsid has an estimated mass of 60 megadaltons and plays an important role in virus stability and infectivity. AAV5 is expressed in various tissues but its expression pattern can differ based on the type of tissue and species being studied.
Biological function summary

The AAV5 capsid facilitates entry into host cells by interacting with cellular receptors. It is not a component of a larger complex but its role is important for delivering genetic material into cells. The virus's ability to transduce a wide range of cell types makes it highly effective in gene therapy applications. This versatility allows researchers to target specific tissues and diseases.

Pathways

AAV5 plays an important role in gene delivery systems which can impact the expression of genetically targeted therapies. Its interaction with heparan sulfate proteoglycans during cellular entry highlights its involvement in pathways related to cellular uptake and transfer of genetic material. This function is highly relevant when considering AAV5 as a tool for introducing therapeutic genes into cells alongside proteins like other adeno-associated viruses which may share similar pathways.

AAV5 has emerged as a powerful tool in the treatment of genetic disorders such as hemophilia and neurodegenerative diseases. Its ability to transduce non-dividing cells makes it suitable for long-term expression in cells. AAV5's interaction with the coagulation factor IX protein can provide therapeutic benefits in hemophilia B patients when delivered effectively. It also shows promise in delivering genes to neural tissues potentially aiding in treating disorders like Parkinson’s disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information AAV5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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