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AB291243

Anti-AAV5 antibody [HL2476] - BSA and Azide free

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Mouse Recombinant Monoclonal AAV5 antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - adeno-associated virus 5 samples.
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Indirect ELISA - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)
  • I-ELISA

Lab

Indirect ELISA - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)

This data was developed using ab315831, the same antibody clone in a different buffer formulation.

Indirect ELISA showing ab315829 binding to AAV8 & AAV3B empty capsid. Binding of ab315829 was assessed in a serial dilution and EC-50 is 1.234 ng/ml for AAV8 and 6.202 ng/ml for AAV3B. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.

Antigen : AAV1 empty capsid,AAV2 empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid AAV3B empty capsid.

Antigen concentration : 7.5E+10 vp/mL

Dot Blot - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)
  • Dot

Supplier Data

Dot Blot - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)

This data was developed using ab315831, the same antibody clone in a different buffer formulation.

Dot blot analysis of AAV5 using ab315831 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

false

Exposure time: 8min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HL2476

Isotype

IgG2b

Carrier free

Yes

Reacts with

adeno-associated virus 5

Applications

Dot, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - adeno-associated virus 5": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" }, "adeno-associated virus 5": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }
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Product details

ab291243 is the carrirer-free version of ab315831.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AAV5 or Adeno-Associated Virus type 5 is a non-pathogenic virus used widely as a vector in gene therapy. This virus has a protein structure named the AAV5 capsid which serves as its protective shell. The AAV5 capsid has an estimated mass of 60 megadaltons and plays an important role in virus stability and infectivity. AAV5 is expressed in various tissues but its expression pattern can differ based on the type of tissue and species being studied.
Biological function summary

The AAV5 capsid facilitates entry into host cells by interacting with cellular receptors. It is not a component of a larger complex but its role is important for delivering genetic material into cells. The virus's ability to transduce a wide range of cell types makes it highly effective in gene therapy applications. This versatility allows researchers to target specific tissues and diseases.

Pathways

AAV5 plays an important role in gene delivery systems which can impact the expression of genetically targeted therapies. Its interaction with heparan sulfate proteoglycans during cellular entry highlights its involvement in pathways related to cellular uptake and transfer of genetic material. This function is highly relevant when considering AAV5 as a tool for introducing therapeutic genes into cells alongside proteins like other adeno-associated viruses which may share similar pathways.

AAV5 has emerged as a powerful tool in the treatment of genetic disorders such as hemophilia and neurodegenerative diseases. Its ability to transduce non-dividing cells makes it suitable for long-term expression in cells. AAV5's interaction with the coagulation factor IX protein can provide therapeutic benefits in hemophilia B patients when delivered effectively. It also shows promise in delivering genes to neural tissues potentially aiding in treating disorders like Parkinson’s disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information AAV5
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