JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315830

Anti-AAV8 + AAV3B antibody [HL2383-IgG1] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Recombinant Monoclonal AAV8 antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Adeno-associated virus 3B, Recombinant fragment - Adeno-associated virus - 8 samples.

View Alternative Names

Adeno-associated virus - 8

2 Images
Indirect ELISA - Anti-AAV8 + AAV3B antibody [HL2383-IgG1] - BSA and Azide free (AB315830)
  • I-ELISA

Lab

Indirect ELISA - Anti-AAV8 + AAV3B antibody [HL2383-IgG1] - BSA and Azide free (AB315830)

This data was developed using ab315829, the same antibody clone in a different buffer formulation.

Indirect ELISA showing ab315829 binding to AAV8 & AAV3B empty capsid. Binding of ab315829 was assessed in a serial dilution and EC-50 is 1.234 ng/ml for AAV8 and 6.202 ng/ml for AAV3B. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.

Antigen : AAV1 empty capsid,AAV2 empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid AAV3B empty capsid.

Antigen concentration : 7.5E+10 vp/mL

Dot Blot - Anti-AAV8 + AAV3B antibody [HL2383-IgG1] - BSA and Azide free (AB315830)
  • Dot

Supplier Data

Dot Blot - Anti-AAV8 + AAV3B antibody [HL2383-IgG1] - BSA and Azide free (AB315830)

This data was developed using ab315829, the same antibody clone in a different buffer formulation.

Dot blot analysis of AAV8 + AAV3B using ab315829 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

true

Exposure time: 8min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HL2383-IgG1

Isotype

IgG1

Carrier free

Yes

Reacts with

Adeno-associated virus - 8, Adeno-associated virus 3B

Applications

I-ELISA, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Adeno-associated virus - 8": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Adeno-associated virus 3B": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Adeno-associated virus - 8": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" }, "Recombinant fragment - Adeno-associated virus 3B": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
style
left-column

Product details

ab315830 is the carrier-free version of ab315829.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adeno-associated virus serotype 8 (AAV8) and AAV3B are variants belonging to the parvovirus family known for their roles as vectors in gene therapy. These serotypes do not have a known mass as they are not proteins but viral capsids made up of proteins. AAV8 and AAV3B are expressed by recombinant technology and utilized as delivery vehicles in various tissues and cells. AAV8 is especially known for its efficiency in transducing liver muscle and retina while AAV3B shows strong affinity for human hepatocytes.
Biological function summary

AAV8 and AAV3B lack replicative properties in the absence of a helper virus making them a safe choice for gene delivery systems. Unlike proteins that are part of a complex these viruses exist independently but rely on the host cellular machinery for successful transduction. Through their distinct tissue tropisms these serotypes enable targeted delivery of genetic material which enhances therapeutic outcomes.

Pathways

AAV8 and AAV3B integrate into host cell processes by hijacking the endosomal trafficking and nuclear import pathways. They remain episomal avoiding integration into the host genome reducing the risk of insertional mutagenesis. AAV3B specifically associates with serotypes like AAV2 in receptor binding and entry pathways which can impact its efficacy and targeting strategy in therapeutic applications.

AAV8 has shown significant potential in liver-targeted therapies particularly in the treatment of hemophilia by delivering functional copies of the factor IX gene. AAV3B on the other hand is under investigation for its role in targeting hepatocellular carcinoma cells due to its affinity for hepatocytes. Collaboration with proteins like the erythroblastic leukemia viral oncogene homolog 2 (ErbB2) aids AAV3B's therapeutic strategies by honing its specificity to cancerous cells.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information AAV8

Additional targets

AAV3B
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com