Mouse Recombinant Monoclonal AAV8 antibody. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Adeno-associated virus 3B, Recombinant fragment - Adeno-associated virus - 8 samples.
Images
![Dot Blot - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (AB315827), expandable thumbnail](https://content.abcam.com/products/images/aav8-aav3b-antibody-hl2383-igg3-ab315827--dot-blot-img427371.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (AB315827)")
![Indirect ELISA - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (AB315827), expandable thumbnail](https://content.abcam.com/products/images/aav8-aav3b-antibody-hl2383-igg3-ab315827--indirect-elisa-img446397.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (AB315827)")
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | Dot | |
|---|---|---|
| Adeno-associated virus - 8 | Predicted | Predicted |
| Adeno-associated virus 3B | Predicted | Predicted |
| Recombinant fragment - Adeno-associated virus - 8 | Tested | Tested |
| Recombinant fragment - Adeno-associated virus 3B | Tested | Tested |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Adeno-associated virus 3B | Dilution info 333.333 ng/mL | Notes - |
Species Recombinant fragment - Adeno-associated virus - 8 | Dilution info 333.333 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Adeno-associated virus - 8, Adeno-associated virus 3B | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Adeno-associated virus 3B | Dilution info 1/2000 | Notes - |
Species Recombinant fragment - Adeno-associated virus - 8 | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Adeno-associated virus - 8, Adeno-associated virus 3B | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Additional Targets
AAV3B
Alternative names
Adeno-associated virus - 8
Mouse Recombinant Monoclonal AAV8 antibody. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Adeno-associated virus 3B, Recombinant fragment - Adeno-associated virus - 8 samples.
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- HL2383-IgG3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adeno-associated virus serotype 8 (AAV8) and AAV3B are variants belonging to the parvovirus family known for their roles as vectors in gene therapy. These serotypes do not have a known mass as they are not proteins but viral capsids made up of proteins. AAV8 and AAV3B are expressed by recombinant technology and utilized as delivery vehicles in various tissues and cells. AAV8 is especially known for its efficiency in transducing liver muscle and retina while AAV3B shows strong affinity for human hepatocytes.
- Biological function summary
AAV8 and AAV3B lack replicative properties in the absence of a helper virus making them a safe choice for gene delivery systems. Unlike proteins that are part of a complex these viruses exist independently but rely on the host cellular machinery for successful transduction. Through their distinct tissue tropisms these serotypes enable targeted delivery of genetic material which enhances therapeutic outcomes.
- Pathways
AAV8 and AAV3B integrate into host cell processes by hijacking the endosomal trafficking and nuclear import pathways. They remain episomal avoiding integration into the host genome reducing the risk of insertional mutagenesis. AAV3B specifically associates with serotypes like AAV2 in receptor binding and entry pathways which can impact its efficacy and targeting strategy in therapeutic applications.
- Associated diseases and disorders
AAV8 has shown significant potential in liver-targeted therapies particularly in the treatment of hemophilia by delivering functional copies of the factor IX gene. AAV3B on the other hand is under investigation for its role in targeting hepatocellular carcinoma cells due to its affinity for hepatocytes. Collaboration with proteins like the erythroblastic leukemia viral oncogene homolog 2 (ErbB2) aids AAV3B's therapeutic strategies by honing its specificity to cancerous cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Dot Blot - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (ab315827), expandable thumbnail](https://content.abcam.com/products/images/aav8-aav3b-antibody-hl2383-igg3-ab315827--dot-blot-img427371.jpg "Dot Blot - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (ab315827)")
Dot Blot - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (ab315827)
AAV8 + AAV3B Dot Blot staining using mouse Anti-AAV8 + AAV3B antibody
Dot blot analysis of AAV8 + AAV3B using ab315827 at 1:2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1:5000 dilution.
Dot blot was performed using 1 ng of each capsid loaded per well.
Anti-AAV9 antibody [HL2368] (Anti-AAV9 antibody [HL2368] ab315818) (1:2000), Anti-AAV9 antibody [HL2370] (Anti-AAV9 antibody [HL2370] ab315820) (1:2000), Anti-AAV9 antibody [HL2374] (Anti-AAV9 antibody [HL2374] ab315823) (1:2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free ab315826) (1:2000) specifically recognize AAV9 capsid.
Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1:2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (Anti-AAV8 + AAV3B antibody [HL2383-IgG1] ab315829) (1:2000) specifically recognize AAV8/3B capsid.
Anti-AAV8 + AAV9 antibody [HL2372] (Anti-AAV8 + AAV9 antibody [HL2372] ab315821) (1:2000) specifically recognizes AAV8/9 capsid.
Anti-AAV5 antibody [HL2476] (Anti-AAV5 antibody [HL2476] ab315831) (1:2000) specifically recognizes AAV5 capsid.
Developed using the ECL technique.
Exposure time: 8min
![Indirect ELISA - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (ab315827), expandable thumbnail](https://content.abcam.com/products/images/aav8-aav3b-antibody-hl2383-igg3-ab315827--indirect-elisa-img446397.jpg "Indirect ELISA - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (ab315827)")
Indirect ELISA - Anti-AAV8 + AAV3B antibody [HL2383-IgG3] (ab315827)
Indirect ELISA showing ab315827 binding to AAV8 & AAV3B empty capsid. Binding of ab315827 was assessed in a serial dilution and EC-50 is 1.799 ng/ml for AAV8. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.
Antigen: AAV1 empty capsid,AAV2 empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid ,AAV3B empty capsid.
Antigen concentration: 7.5E+10 vp/mL
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com