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AB315822

Anti-AAV8 + AAV9 antibody [HL2372] - BSA and Azide free

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Mouse Recombinant Monoclonal AAV8 antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Adeno-associated virus - 8, Recombinant fragment - Adeno-associated virus 9 samples.

View Alternative Names

Adeno-associated virus - 8

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Dot Blot - Anti-AAV8 + AAV9 antibody [HL2372] - BSA and Azide free (AB315822)
  • Dot

Supplier Data

Dot Blot - Anti-AAV8 + AAV9 antibody [HL2372] - BSA and Azide free (AB315822)

This data was developed using ab315821, the same antibody clone in a different buffer formulation.

Dot blot analysis of AAV8 + AAV9 using ab315821 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

true

Exposure time: 8min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HL2372

Isotype

IgG2a

Carrier free

Yes

Reacts with

Adeno-associated virus - 8, Adeno-associated virus 9

Applications

I-ELISA, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Adeno-associated virus - 8": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Adeno-associated virus 9": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Adeno-associated virus - 8": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" }, "Recombinant fragment - Adeno-associated virus 9": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
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Product details

ab315822 is the carrier-free version of ab315821.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adeno-associated virus serotypes AAV8 and AAV9 are types of non-enveloped viruses. They belong to the Parvoviridae family and show varied capsid (protein shell) structures which influence their host interactions and applications in gene therapy. The mass of the AAV capsid is approximately 25 kilodaltons. These viruses target a wide range of tissues with AAV8 showing strong tropism for liver tissue and AAV9 having extensive distribution including the central nervous system and cardiac tissues. In experimental settings AAV variants are frequently used due to their efficiency and safety in delivering genetic material into cells for gene therapy.
Biological function summary

AAV8 and AAV9 are versatile vectors that carry therapeutic genes to specific cells without integrating into the host genome. They are not known to cause any human disease which makes them attractive in clinical applications. AAV9 for instance can cross the blood-brain barrier allowing it to reach neurological targets efficiently. Although these vectors do not function as part of a larger biological complex their ability to encapsulate expression cassettes distinguishes them from other viral vectors enhancing their use in targeting diverse genetic disorders.

Pathways

AAV8 and AAV9 are critical tools in gene transfer technology leveraging various mechanisms such as endocytosis to enter target cells. They primarily participate in pathways involving the transduction of encoded genetic material avoiding integration into the host DNA. AAV8 has displayed efficient interaction with hepatic pathways while AAV9 is linked with neuronal pathways due to its ability to cross the blood-brain barrier. These serotypes potentially interact with specific cellular receptors or transport proteins during their entry and trafficking within host cells facilitating targeted gene delivery.

AAV8 and AAV9 have become prominent in studies targeting genetic conditions like muscular dystrophy and spinal muscular atrophy. AAV9's ability to penetrate neural tissues makes it a valuable vector for treating neurological disorders such as these. Additionally AAV8 is often used in liver-related diseases where its strong liver-targeting properties play a significant role. The effectiveness of these serotypes in delivering genes to specific tissues has solidified their role in therapeutic strategies working in conjunction with proteins involved in the pathogenesis of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information AAV8

Additional targets

AAV9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com