AB291244

Anti-AAV9 antibody [HL2370] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal AAV9 antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Adeno-associated virus 9 samples.

View Alternative Names

Adeno-associated virus - 9

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Indirect ELISA - Anti-AAV9 antibody [HL2370] - BSA and Azide free (AB291244)

I-ELISA

Lab

Indirect ELISA - Anti-AAV9 antibody [HL2370] - BSA and Azide free (AB291244)

This data was developed using ab315820, the same antibody clone in a different buffer formulation.

Indirect ELISA showing ab315820 binding to AAV9 empty capsid. Binding of ab315820 was assessed in a serial dilution and EC-50 is 2.441 ng/ml. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.

Antigen : AAV1 empty capsid,AAV2 empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid,AAV3B empty capsid.

Antigen concentration : 7.5E+10 vp/mL

Supplier Data

Dot Blot - Anti-AAV9 antibody [HL2370] - BSA and Azide free (AB291244)

This data was developed using ab315820, the same antibody clone in a different buffer formulation.

Dot blot analysis of AAV9 using ab315820 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

true

Exposure time: 8min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HL2370

Isotype

IgG2a

Carrier free

Yes

Reacts with

Adeno-associated virus 9

Applications

I-ELISA, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Adeno-associated virus 9": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Adeno-associated virus 9": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }

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Product details

ab291244 is the carrier-free version of ab315820.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adeno-associated virus serotype 9 (AAV9) is a viral vector used in gene therapy known for its ability to transduce cells by delivering genetic material. Popular as a genetic tool AAV9 is utilized because of its efficient transfection capabilities in a variety of tissues. It consists of a protein capsid which forms an icosahedral structure important for its ability to encapsulate and deliver therapeutic DNA. The AAV9 capsid has an approximate molecular mass of 60 kilodaltons per subunit assembling into a larger capsid particle. AAV9 exhibits a broad expression profile being able to transduce diverse tissues including muscle liver central nervous system and cardiac tissues.

Biological function summary

AAV9 targets enable the efficient delivery of genes by trafficking across the cellular barriers leading to gene expression within target cells. Part of the larger family of adeno-associated viruses AAV9's specificity for different tissues allows researchers to target certain organs for therapeutic purposes. The AAV9 capsid engages with cell surface receptors and undergoes internalization by endocytosis. Interestingly different serotypes within this family vary slightly in tissue tropism and selection of the appropriate serotype affects therapeutic outcomes.

Pathways

AAV9 interacts with cellular regulatory mechanisms involved in transporting genetic material to the nucleus where it integrates into the genome or exists episomally. It is notably applied in pathways related to muscle and CNS diseases where AAV9 enhances the delivery of therapeutic genes. It operates in conjunction with certain cellular proteins that facilitate endosomal escape and nuclear entry making it effective for addressing genetic defects linked with these pathways.

AAV9 has been linked to therapeutic strategies for conditions such as spinal muscular atrophy (SMA) and certain types of muscular dystrophy. Utilization of AAV9 allows for the delivery of corrective genes potentially reversing or halting disease progression. In SMA for example AAV9 is used to deliver survival motor neuron (SMN) gene which is deficient in patients. Similarly in muscular dystrophy AAV9 aids in the administration of dystrophin or microdystrophin genes which play an important role in muscle integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information AAV9

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AAV9 antibody [HL2370] - BSA and Azide free

Indirect ELISA - Anti-AAV9 antibody [HL2370] - BSA and Azide free (AB291244)

Dot Blot - Anti-AAV9 antibody [HL2370] - BSA and Azide free (AB291244)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise