Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

Rabbit Recombinant Monoclonal ABAT/GABA-T antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (AB233702), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	IHC-Fr	Flow Cyt (Intra)	IHC-P
Human	Tested	Expected	Tested	Expected	Tested	Tested
Mouse	Expected	Expected	Expected	Tested	Expected	Expected
Rat	Predicted	Expected	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information ABAT

Function

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively (PubMed:10407778, PubMed:15528998). Can also convert delta-aminovalerate and beta-alanine (By similarity).

Alternative names

GABAT, ABAT, (S)-3-amino-2-methylpropionate transaminase, GABA aminotransferase, Gamma-amino-N-butyrate transaminase, L-AIBAT, GABA-AT, GABA transaminase, GABA-T

Recommended products

Rabbit Recombinant Monoclonal ABAT/GABA-T antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR20842
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab233702 is the carrier-free version of Anti-ABAT/GABA-T antibody [EPR20842] ab216465.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ABAT also known as GABA transaminase (GABA-T) is an enzyme involved in the catabolism of the neurotransmitter gamma-aminobutyric acid (GABA). This enzyme catalyzes the conversion of GABA to succinic semialdehyde an important step in the GABA degradation pathway. GABA-T has a molecular mass of approximately 56 kDa. Its expression primarily occurs in the brain kidney and liver where it regulates GABA levels influencing neurological functions.

Biological function summary

ABAT plays a critical role in maintaining GABA homeostasis in the central nervous system. The protein functions as a homodimer forming a complex that effectively binds to its substrate. It modulates the inhibitory effects mediated by GABA in neuronal signaling. This regulation is essential for processes such as synaptic transmission and plasticity impacting a range of neurological activities.

Pathways

ABAT is integral to the GABAergic neurotransmission pathway which balances excitatory and inhibitory signals in the brain. It operates in conjunction with glutamate decarboxylase another enzyme playing an important role in GABA synthesis. Together they maintain the dynamic equilibrium between GABA production and breakdown. Additionally the enzyme interacts with proteins that contribute to the tricarboxylic acid cycle linking neurotransmitter metabolism to energy production.

Associated diseases and disorders

ABAT is associated with several neurological conditions including epilepsy and schizophrenia. Impaired function or expression of ABAT can lead to altered GABA levels contributing to the pathophysiology of these disorders. The enzyme also connects with GABA receptor subtypes which are targets for therapeutic interventions aimed at modulating GABAergic activity to address these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
ABAT/GABA-T was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/5000 dilution.
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).
Lane 2: Anti-ABAT/GABA-T antibody [EPR20842] ab216465 IP in MCF7 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ABAT/GABA-T antibody [EPR20842] ab216465 in MCF7 whole cell lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
All lanes: Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR20842] (Anti-ABAT/GABA-T antibody [EPR20842] ab216465)
Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunofluorescent analysis of 100% methanol-fixed HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 (green). Confocal image showing mitochondrial staining in HepG2 cell line is observed. The nuclear counter stain is DAPI (blue).
Mitochondria are stained with Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor^®594 Goat anti-Mouse secondary both at 1/1000 dilution (red).
-ve control 1: Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (AlexaFluor®488 Goat anti-Rabbit secondary) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunofluorescent analysis of 100% methanol-fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 (green). Confocal image showing mitochondrial staining in MCF7 cell line. The nuclear counter stain is DAPI (blue).
Mitochondria are stained with Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor^®594 Goat anti-Mouse secondary both at 1/1000 dilution (red).
-ve control 1: Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (AlexaFluor®488 Goat anti-Rabbit secondary) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/600 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in rat liver is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat choroid plexus tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in rat choroid plexus (PMID:25239459, PMID: 11459221) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse choroid plexus tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in mouse choroid plexus (PMID:25239459, PMID: 11459221) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in mouse cerebellum (PMID:25239459, PMID: 11459221) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in rat liver (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in mouse testis (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in human breast cancer (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in human kidney (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).
Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling ABAT/GABA-T with Anti-ABAT/GABA-T antibody [EPR20842] ab216465 at 1/60 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ABAT/GABA-T antibody [EPR20842] ab216465).