Anti-ABCA1 antibody [AB.H10] (ab18180) is a mouse monoclonal antibody that is used to detect ABCA1 in Western Blot, Flow Cytometry, IHC-P. Suitable for Human, Mouse samples.
- Specificity confirmed with ABCA1 knockout cell line validation
Images
![Western blot - Anti-ABCA1 antibody [AB.H10] (AB18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--western-blot-img8528.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [AB.H10] (AB18180)")
![Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (AB18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--flow-cytometry-img50556.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (AB18180)")
![Western blot - Anti-ABCA1 antibody [AB.H10] (AB18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--western-blot-img8267.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [AB.H10] (AB18180)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (AB18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46579.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (AB18180)")
Publications
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- International journal of molecular sciences 24:2023MP Allosterically Activates AMPK to Enhance ABCA1 Stability by Retarding the Calpain-Mediated Degradation Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Li,Mingchao Wang,Kai Qu,Ruiming Xu,Haibo ZhuPubMed 38139111
- Nutrients 15:2023Impact of Ring Finger Protein 20 and Its Downstream Regulation on Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a High-Fat Diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Jin Kim,Se-Hyun Oh,Jeong-Hoon Lim,Jang-Hee Cho,Hee-Yeon Jung,Chan-Duck Kim,Sun-Hee Park,Tae-Hwan Kwon,Yong-Lim KimPubMed 38068817
- Life science alliance 6:2023Macrophage promotes pathological neovascularization in age-related macular degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYafang Wang,Yang Liu,Yan Wang,Yidong Wu,Zhixuan Chen,Feng Wang,Xiaoling Wan,Fenghua Wang,Xiaodong SunPubMed 37550000
- Communications biology 6:7892023An iPSC-derived astrocyte model of fragile X syndrome exhibits dysregulated cholesterol homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaro Talvio,Victoria A Wagner,Rimante Minkeviciene,Jay S Kirkwood,Anna O Kulinich,Juzoh Umemori,Anil Bhatia,Manhoi Hur,Reijo Käkelä,Iryna M Ethell,Maija L CastrénPubMed 37516746
- Cell biology international 47:1589-15992023Macrophage Gpx4 deficiency aggravates foam cell formation by regulating the expression of scavenger receptors, ABCA1, and ABCG1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingquan Zhou,Suhua Wu,Xiaoqin Chen,Lianjie Hou,Qiong Zhong,Weixia Luo,Chunni Dai,Xiaoyan DaiPubMed 37309064
- Nutrients 15:2023Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyan Liu,Yuan Zhang,Xiangyu Zheng,Ziling Wang,Pan Wang,Mengdi Zhang,Mengfan Shen,Yongping Bao,Dan LiPubMed 37432260
- Cells 12:2023Liver X Receptor Activation Attenuates Oxysterol-Induced Inflammatory Responses in Fetoplacental Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeekha George,Magdalena Lang,Chaitanya Chakravarthi Gali,Joshua Adekunle Babalola,Carmen Tam-Amersdorfer,Anika Stracke,Herbert Strobl,Robert Zimmermann,Ute Panzenboeck,Christian WadsackPubMed 37190095
- International journal of molecular medicine 51:2023STAT3/NF‑κB decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑κB signaling pathway in a mouse model of atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun-Jin An,Mi-Gyeong Gwon,Hyemin Gu,Seongjae Bae,Jaechan Leem,Jin Bae Lee,Kwan-Kyu ParkPubMed 37026512
- Nutrients 15:2023Effects of Caprylic Acid and Eicosapentaenoic Acid on Lipids, Inflammatory Levels, and the JAK2/STAT3 Pathway in ABCA1-Deficient Mice and ABCA1 Knock-Down RAW264.7 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinsheng Zhang,Peng Zhang,Yinghua Liu,Zhao Liu,Qing Xu,Yong Zhang,Lu Liu,Xueyan Yang,Liya Li,Changyong XuePubMed 36904298
- Journal of translational medicine 21:972023Genetic dissection of the impact of lncRNA AI662270 during the development of atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Hong,Yue Zhang,Hui Chen,Xueqing Tang,Hongrui Zhao,Ziyu Meng,Xueling Jia,Wenfeng Liu,Xiaohan Li,Lin Wang,Xinrui Zhong,Xuefeng Bai,Heyang Sun,Philipp Kopylov,Bestavashvili Afina,Dmitry Shchekochikhin,Yong Zhang,Xin Liu,Yuhua FanPubMed 36755320
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ABCA1 aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95477
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Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200.00000 - 1/500.00000 | Notes Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate. |
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019).
Alternative names
ABC1, CERP, ABCA1, Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1
Recommended products
Anti-ABCA1 antibody [AB.H10] (ab18180) is a mouse monoclonal antibody that is used to detect ABCA1 in Western Blot, Flow Cytometry, IHC-P. Suitable for Human, Mouse samples.
- Specificity confirmed with ABCA1 knockout cell line validation
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ABCA1 aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95477
- Clone number
- AB.H10
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Protein A purified from TCS
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ABCA1 antibody [AB.H10] (ab18180) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of ABCA1?
Anti-ABCA1 [AB.H10] (ab18180) specifically detects a band for ABCA1 (UniProt: O95477) at a molecular weight of 254kDa.
Trusted by the scientific community
Anti-ABCA1 [AB.H10] (ab18180) was first used in a scientific publication in 2005 and has been cited over 200 times in peer-reviewed journals.
Reviewed by scientists
Anti-ABCA1 [AB.H10] (ab18180) has over 15 independent reviews from customers.
Specificity confirmed
The specificity of Anti-ABCA1 antibody [AB.H10] (ab18180) has been confirmed by Western blot testing in ABCA1 Knockout Mouse liver samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.
- Biological function summary
ABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.
- Pathways
Many regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.
- Associated diseases and disorders
ABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
![Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--western-blot-img8528.jpg "Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)")
Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.
All lanes: Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
Predicted band size: 254 kDa
![Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--flow-cytometry-img50556.jpg "Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)")
Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)
Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
![Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--western-blot-img8267.jpg "Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)")
Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
The lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.
All lanes: Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution
Lanes 1, 3 and 5: Wild type mouse liver tissue lysate at 100 µg
Lanes 2, 4 and 6: Knockout mouse liver tissue lysate at 100 µg
Predicted band size: 254 kDa
Observed band size: 254 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-abh10-ab18180--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46579.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)
Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.
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