Rabbit Recombinant Monoclonal ABCA1 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--western-blot-img428632.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535)")
![Immunoprecipitation - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--immunoprecipitation-img406388.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535)")
![Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--western-blot-img405385.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535)")
![Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--western-blot-img405384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535)")
![Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--immunocytochemistry-immunofluorescence-img405382.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (AB307535)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein. |
Species Mouse | Dilution info - | Notes We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein. |
Target data
Function
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019).
Alternative names
ABC1, CERP, ABCA1, Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1
Recommended products
Rabbit Recombinant Monoclonal ABCA1 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27494-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.
- Biological function summary
ABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.
- Pathways
Many regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.
- Associated diseases and disorders
ABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--western-blot-img428632.jpg "Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)")
Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)
This data was developed using Anti-ABCA1 antibody [EPR27494-51] ab307534, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The higher band is probably the glycosylated form (PMID: 16873719).
All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-51] (Anti-ABCA1 antibody [EPR27494-51] ab307534) at 1/1000 dilution
Lane 1: Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 2: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg
Lane 3: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HepG2 treated with 20uM T0901317 for 16 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 254 kDa
Observed band size: 254 kDa, 400 kDa
Exposure time: 10s
![Immunoprecipitation - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--immunoprecipitation-img406388.jpg "Immunoprecipitation - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)")
Immunoprecipitation - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)
This data was developed using Anti-ABCA1 antibody [EPR27494-51] ab307534, the same antibody clone in a different buffer formulation.
ABCA1 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 10 ug with Anti-ABCA1 antibody [EPR27494-51] ab307534 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ABCA1 antibody [EPR27494-51] ab307534 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 10 ug
Lane 2: ABAB307534 IP in U-87 MG whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ABCA1 antibody [EPR27494-51] ab307534 in U-87 MG whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 secondsAll lanes: Immunoprecipitation - Anti-ABCA1 antibody [EPR27494-51] (Anti-ABCA1 antibody [EPR27494-51] ab307534) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 10 μg
Lane 2: U-87 MG whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 260 kDa
Exposure time: 24s
![Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--western-blot-img405385.jpg "Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)")
Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)
This data was developed using 307534, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: 293T(PMID:11896206), SH-SY5Y(PMID: 23600914).
Bands below 260 kDa may due to degradation.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time:All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-51] (Anti-ABCA1 antibody [EPR27494-51] ab307534) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 40 μg
Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 40 μg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate 40 μg
Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 40 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 26s
![Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--western-blot-img405384.jpg "Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)")
Western blot - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)
This data was developed using 307534, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Samples are non-boiled as boiling may cause protein aggregates.
This antibody does not cross-react with human ABCA3, ABCA4 and ABCA7.
3 seconds
Exposure time:All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-51] (Anti-ABCA1 antibody [EPR27494-51] ab307534) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 2: 293T cells transfected with an empty vector containi a myc-his tag whole cell lysate 20 μg
Lane 3: 293T cells transfected with a human CA1 expression vector containi a myc-his tag whole cell lysate 10 μg
Lane 4: 293T cells transfected with a human CA3 expression vector containi a myc-his tag whole cell lysate 36 μg
Lane 5: 293T cells transfected with a human CA4 expression vector containi a myc-his tag whole cell lysate 4 μg
Lane 6: 293T cells transfected with a human CA7 expression vector containi a myc-his tag whole cell lysate 4 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 3s
![Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-epr27494-51-bsa-and-azide-free-ab307535--immunocytochemistry-immunofluorescence-img405382.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)")
Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 antibody [EPR27494-51] - BSA and Azide free (ab307535)
This data was developed using Anti-ABCA1 antibody [EPR27494-51] ab307534, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells lABelling ABCA1 with Anti-ABCA1 antibody [EPR27494-51] ab307534 at 1/500 (1.042 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in U-87 MG cell line.Negative control: 293T (PMID:11896206).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/ml dilution.
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