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Rabbit Recombinant Monoclonal ABCA1 antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536), expandable thumbnail
  • Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536), expandable thumbnail
  • Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536), expandable thumbnail
  • Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IFIHC-FrIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.

Species
Mouse
Dilution info
1/1000
Notes

We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.

Target data

Function

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019).

Alternative names

ABC1, CERP, ABCA1, Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1

Recommended products

Rabbit Recombinant Monoclonal ABCA1 antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27494-57
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.

Biological function summary

ABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.

Pathways

Many regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.

Associated diseases and disorders

ABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure: lanes 1-4: 180 seconds; lanes: 5-8; 10 seconds

    The higher band is probably glycosylated form (PMID: 16873719).

    Compared with ab307536, Anti-ABCA1 antibody [EPR27494-51] ab307534 has higher sensitivity.

    We recommend Anti-ABCA1 antibody [EPR27494-51] ab307534 as an alternative in testing human lysate, or optimize experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to get visible band.

    Lanes 1 - 4: Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536) at 1/1000 dilution

    Lanes 5 - 8: Western blot - Anti-ABCA1 antibody [EPR27494-51] (Anti-ABCA1 antibody [EPR27494-51] ab307534) at 1/1000 dilution

    Lane 6: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg

    Lane 7: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 1 and 5: Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg

    Lane 3: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 4 and 8: HepG2 treated with 20uM T0901317 for 16 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 254 kDa

    Observed band size: 254 kDa, 400 kDa

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTABCA1 expression was increased after treatment with the nonsteroidal LXR agonist T0901317 (PMID: 12384498).

    Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206).

    Samples are non-boiled as boiling may cause protein aggregates.
    Exposure time: Lanes 1: 37 seconds, Lane 2 and 3: 103 seconds.

    All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate 50 μg

    Lane 2: Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 40 μg

    Lane 3: Neuro-2a treated with 1uM T0901317 for 24 hours whole cell lysate 40 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 260 kDa

    Exposure time: 37s

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: 293T(PMID:11896206), SH-SY5Y(PMID: 23600914)

    Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206). Bands below 260 kDa may due to degradation.

    Samples are non-boiled as boiling may cause protein aggregates.
    Exposure time: 48 seconds

    All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 40 μg

    Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 40 μg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate 40 μg

    Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 40 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 260 kDa

    Exposure time: 48s

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis antibody does not cross-react with human ABCA3, ABCA4 and ABCA7.

    Samples are non-boiled as boiling may cause protein aggregates.


    Exposure time: 15 seconds

    All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (ab307536) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

    Lane 2: 293T cells transfected with an empty vector containi a myc-his tag whole cell lysate 20 μg

    Lane 3: 293T cells transfected with a human CA1 expression vector containi a myc-his tag whole cell lysate 10 μg

    Lane 4: 293T cells transfected with a human CA3 expression vector containi a myc-his tag whole cell lysate 36 μg

    Lane 5: 293T cells transfected with a human CA4 expression vector containi a myc-his tag whole cell lysate 4 μg

    Lane 6: 293T cells transfected with a human CA7 expression vector containi a myc-his tag whole cell lysate 4 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 260 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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