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Rabbit Recombinant Monoclonal ABCA1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IFIHC-FrIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.

Species

Mouse

Dilution info

-

Notes

We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein.

Target data

Function

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981). Thereby, participates in phospholipid transfer to apoliproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apoliproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981).

Alternative names

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Rabbit Recombinant Monoclonal ABCA1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EPR27494-57

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537)

    This data was developed using ab307536, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure: lanes 1-4: 180 seconds; lanes: 5-8; 10 seconds

    The higher band is probably glycosylated form (PMID: 16873719).

    Compared with ab307536, ab307534 has higher sensitivity.

    We recommend ab307534 as an alternative in testing human lysate, or optimize experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to get visible band.

    Lanes 1 - 4: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution

    Lanes 5 - 8: Western blot - Anti-ABCA1 antibody [EPR27494-51] (AB307534) at 1/1000 dilution

    Lane 6: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg

    Lane 7: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 1 and 5: Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg

    Lane 3: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 4 and 8: HepG2 treated with 20uM T0901317 for 16 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 254 kDa

    Observed band size: 254 kDa, 400 kDa

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure: lanes 1-4: 180 seconds; lanes: 5-8; 10 seconds

    The higher band is probably glycosylated form (PMID: 16873719).

    Compared with ab307536, ab307534 has higher sensitivity.

    We recommend ab307534 as an alternative in testing human lysate, or optimize experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to get visible band.

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537)

    This data was developed using 307536, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: ABCA1 expression was increased after treatment with the nonsteroidal LXR agonist T0901317 (PMID: 12384498).

    Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206).

    Samples are non-boiled as boiling may cause protein aggregates.
    Lanes 1: 37 seconds, Lane 2 and 3: 103 seconds.
    Exposure time:

    All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate 50 μg

    Lane 2: Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 40 μg

    Lane 3: Neuro-2a treated with 1uM T0901317 for 24 hours whole cell lysate 40 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 260 kDa

    Exposure time: 37s

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTABCA1 expression was increased after treatment with the nonsteroidal LXR agonist T0901317 (PMID: 12384498).

    Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206).

    Samples are non-boiled as boiling may cause protein aggregates.
    Exposure time: Lanes 1: 37 seconds, Lane 2 and 3: 103 seconds.

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537)

    This data was developed using 307536, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: Negative control: 293T(PMID:11896206), SH-SY5Y(PMID: 23600914)

    Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206). Bands below 260 kDa may due to degradation.

    Samples are non-boiled as boiling may cause protein aggregates.
    48 seconds
    Exposure time:

    All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 40 μg

    Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 40 μg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate 40 μg

    Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 40 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 260 kDa

    Exposure time: 48s

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: 293T(PMID:11896206), SH-SY5Y(PMID: 23600914)

    Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206). Bands below 260 kDa may due to degradation.

    Samples are non-boiled as boiling may cause protein aggregates.
    Exposure time: 48 seconds

  • Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537), expandable thumbnail

    Western blot - Anti-ABCA1 antibody [EPR27494-57] - BSA and Azide free (ab307537)

    This data was developed using 307536, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: This antibody does not cross-react with human ABCA3, ABCA4 and ABCA7.

    Samples are non-boiled as boiling may cause protein aggregates.


    15 seconds
    Exposure time:

    All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

    Lane 2: 293T cells transfected with an empty vector containi a myc-his tag whole cell lysate 20 μg

    Lane 3: 293T cells transfected with a human CA1 expression vector containi a myc-his tag whole cell lysate 10 μg

    Lane 4: 293T cells transfected with a human CA3 expression vector containi a myc-his tag whole cell lysate 36 μg

    Lane 5: 293T cells transfected with a human CA4 expression vector containi a myc-his tag whole cell lysate 4 μg

    Lane 6: 293T cells transfected with a human CA7 expression vector containi a myc-his tag whole cell lysate 4 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 260 kDa

    Exposure time: 15s

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis antibody does not cross-react with human ABCA3, ABCA4 and ABCA7.

    Samples are non-boiled as boiling may cause protein aggregates.


    Exposure time: 15 seconds

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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