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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal ABCA1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IP | Flow Cyt | ICC/IF | IHC-Fr | IHC-P | WB | |
---|---|---|---|---|---|---|
Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein. |
Species Mouse | Dilution info - | Notes We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes We recommend not to boil your lysates before loading to gel when preparing membrane used for western blot. Boiling leads to the formation of aggregates of membrane proteins, leading to unsuccessful migration in gel. Please do not boil your lysate when testing membrane protein. |
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981). Thereby, participates in phospholipid transfer to apoliproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apoliproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981).
Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1, CERP, ABC1, ABCA1
Rabbit Recombinant Monoclonal ABCA1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1, CERP, ABC1, ABCA1
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
Yes
EPR27494-57
Affinity purification Protein A
Blue Ice
+4°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using ab307536, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure: lanes 1-4: 180 seconds; lanes: 5-8; 10 seconds
The higher band is probably glycosylated form (PMID: 16873719).
Compared with ab307536, ab307534 has higher sensitivity.
We recommend ab307534 as an alternative in testing human lysate, or optimize experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to get visible band.
Lanes 1 - 4: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution
Lanes 5 - 8: Western blot - Anti-ABCA1 antibody [EPR27494-51] (AB307534) at 1/1000 dilution
Lane 6: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg
Lane 7: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 1 and 5: Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 2: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg
Lane 3: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 4 and 8: HepG2 treated with 20uM T0901317 for 16 hours whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 254 kDa
Observed band size: 254 kDa, 400 kDa
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure: lanes 1-4: 180 seconds; lanes: 5-8; 10 seconds
The higher band is probably glycosylated form (PMID: 16873719).
Compared with ab307536, ab307534 has higher sensitivity.
We recommend ab307534 as an alternative in testing human lysate, or optimize experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to get visible band.
This data was developed using 307536, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: ABCA1 expression was increased after treatment with the nonsteroidal LXR agonist T0901317 (PMID: 12384498).
Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206).
Samples are non-boiled as boiling may cause protein aggregates.
Lanes 1: 37 seconds, Lane 2 and 3: 103 seconds.
Exposure time:
All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate 50 μg
Lane 2: Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 40 μg
Lane 3: Neuro-2a treated with 1uM T0901317 for 24 hours whole cell lysate 40 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 37s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTABCA1 expression was increased after treatment with the nonsteroidal LXR agonist T0901317 (PMID: 12384498).
Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: Lanes 1: 37 seconds, Lane 2 and 3: 103 seconds.
This data was developed using 307536, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: 293T(PMID:11896206), SH-SY5Y(PMID: 23600914)
Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206). Bands below 260 kDa may due to degradation.
Samples are non-boiled as boiling may cause protein aggregates.
48 seconds
Exposure time:
All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 40 μg
Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 40 μg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate 40 μg
Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 40 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 48s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: 293T(PMID:11896206), SH-SY5Y(PMID: 23600914)
Bands around 500 kDa may due to aggregation (PMID:31641056, PMID: 29103206). Bands below 260 kDa may due to degradation.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 48 seconds
This data was developed using 307536, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: This antibody does not cross-react with human ABCA3, ABCA4 and ABCA7.
Samples are non-boiled as boiling may cause protein aggregates.
15 seconds
Exposure time:
All lanes: Western blot - Anti-ABCA1 antibody [EPR27494-57] (AB307536) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 2: 293T cells transfected with an empty vector containi a myc-his tag whole cell lysate 20 μg
Lane 3: 293T cells transfected with a human CA1 expression vector containi a myc-his tag whole cell lysate 10 μg
Lane 4: 293T cells transfected with a human CA3 expression vector containi a myc-his tag whole cell lysate 36 μg
Lane 5: 293T cells transfected with a human CA4 expression vector containi a myc-his tag whole cell lysate 4 μg
Lane 6: 293T cells transfected with a human CA7 expression vector containi a myc-his tag whole cell lysate 4 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 15s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis antibody does not cross-react with human ABCA3, ABCA4 and ABCA7.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 15 seconds
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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