Mouse Monoclonal ABCA1 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--western-blot-img101366.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33160.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118038.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550)")
![Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--western-blot-img433168.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550)")
![Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--western-blot-img433169.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (AB264550)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.2 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg for 106 Cells | Notes (methanol fixed cells) ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Abcam recommends using 3% Milk as the blocking agent. |
Species Rat | Dilution info 1 µg/mL | Notes Abcam recommends using 3% Milk as the blocking agent. |
Species Human | Dilution info 1 µg/mL | Notes Abcam recommends using 3% Milk as the blocking agent. |
Associated Products
Select an associated product type
2 products for Alternative Product
1 product for Alternative Version
Target data
Function
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019).
Alternative names
ABC1, CERP, ABCA1, Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1
Recommended products
Mouse Monoclonal ABCA1 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- HJ1
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab264550 is the carrier-free version of Anti-ABCA1 antibody [HJ1] ab66217.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.
- Biological function summary
ABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.
- Pathways
Many regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.
- Associated diseases and disorders
ABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--western-blot-img101366.jpg "Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)")
Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: Empty
Lane 3: ABCA1 knockout (KO) HAP1 whole cell lysate (20 μg)Lanes 1 - 3: Merged signal (red and green). Green - Anti-ABCA1 antibody [HJ1] ab66217 observed at 240 kDa. Red - loading control, Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560, observed at 50 kDa.
Anti-ABCA1 antibody [HJ1] ab66217 detected the expected band for ABCA1 in wild type HAP1 cells and the band was not seen in ABCA1 knockout HAP1 cells, although additional non-specific bands were also seen. Wild-type and ABCA1 knockout samples were subjected to SDS-PAGE. Anti-ABCA1 antibody [HJ1] ab66217 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab264550).
All lanes: Western blot - Anti-ABCA1 antibody [HJ1] (Anti-ABCA1 antibody [HJ1] ab66217)
Predicted band size: 254 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33160.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)
IHC image of ABCA1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-ABCA1 antibody [HJ1] ab66217, 0.2μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab264550).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118038.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)
Anti-ABCA1 antibody [HJ1] ab66217 (4μg/ml) staining ABCA1 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is moderate cell membrane staining throughout the liver parenchyma.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab264550).
![Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--western-blot-img433168.jpg "Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)")
Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ABCA1 antibody [HJ1] ab66217).
Blocking and diluting buffer: 5% NFDM/TBST.
Anti-Vinculin antibody [EPR8185] ab129002 was used as a loading control at 1/10000 dilution, followed by a secondary antibody Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/20000 dilution.
Negative control: 293T (PMID:11896206), SH-SY5Y (PMID: 23600914).
Samples are non-boiled as boiling may cause protein aggregates.
All lanes: Western blot - Anti-ABCA1 antibody [HJ1] (Anti-ABCA1 antibody [HJ1] ab66217) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Lanes 1 - 3: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 254 kDa
Observed band size: 254 kDa
Exposure time: 10s
![Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550), expandable thumbnail](https://content.abcam.com/products/images/abca1-antibody-hj1-bsa-and-azide-free-ab264550--western-blot-img433169.jpg "Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)")
Western blot - Anti-ABCA1 antibody [HJ1] - BSA and Azide free (ab264550)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ABCA1 antibody [HJ1] ab66217).
Blocking and diluting buffer: 5% NFDM/TBST.
Anti-Vinculin antibody [EPR8185] ab129002 was used as a loading control at 1/10000 dilution, followed by a secondary antibody Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/20000 dilution.
The higher band is probably glycosylated form (PMID: 16873719).
All lanes: Western blot - Anti-ABCA1 antibody [HJ1] (Anti-ABCA1 antibody [HJ1] ab66217) at 1/1000 dilution
Lane 1: Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 2: THP-1 treated first with 100ng/ml PAM for 72 hours and then replaced with 100ng/ml LPS for 5 hours, 300ng/ml BFA was then added for additional 3 hours whole cell lysate at 20 µg
Lane 3: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HepG2 treated with 20uM T0901317 for 16 hours whole cell lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Lanes 1 - 3: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 254 kDa
Observed band size: 254 kDa, 400 kDa
Exposure time: 20s
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