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AB99953

Anti-ABCA5 antibody

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(3 Publications)

Rabbit Polyclonal ABCA5 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCA5 aa 1450 to C-terminus.

View Alternative Names

KIAA1888, ABCA5, Cholesterol transporter ABCA5, ATP-binding cassette sub-family A member 5

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA5 antibody (AB99953)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA5 antibody (AB99953)

ab99953 at 1/100 dilution, staining ABCA5 in human fetal kidney tissue.

Western blot - Anti-ABCA5 antibody (AB99953)
  • WB

Unknown

Western blot - Anti-ABCA5 antibody (AB99953)

All lanes:

Western blot - Anti-ABCA5 antibody (ab99953) at 1/500 dilution

All lanes:

Human fetal testis lysate

Predicted band size: 187 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ABCA5 aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WWZ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCA5 also known as ATP-binding cassette sub-family A member 5 functions as a transporter protein. It belongs to the ABC transporter family which utilizes ATP hydrolysis to translocate substrates across cellular membranes. The mass of ABCA5 is approximately 183 kDa. This protein is expressed in various tissues including the brain and testes where it plays a role in cellular lipid transport processes.
Biological function summary

The ABCA5 protein impacts lipid metabolism and the maintenance of cellular lipid homeostasis. The protein is not known to be part of any complex but it actively contributes to the movement of cholesterol and other lipid molecules out of cells affecting their availability and distribution. This process supports cellular function and integrity by managing lipid storage and mobilization.

Pathways

ABCA5 plays a significant role in lipid transport and metabolism pathways and cholesterol efflux. It interacts with proteins such as ABCA1 which is also part of the ABC transporter family and facilitates the removal of cholesterol from cells impacting cholesterol homeostasis. These interactions are vital for the proper functioning of lipid management pathways.

Under-functioning or mutations in ABCA5 can associate with lipid storage disorders impacting cellular and systemic lipid regulations. The protein has connections to conditions such as Niemann-Pick disease where lipid accumulation occurs due to disrupted transport. Protein interactions between ABCA5 and proteins like NPC1 involved in Niemann-Pick disease offer insights into the disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cholesterol efflux transporter in macrophages that is responsible for APOAI/high-density lipoproteins (HDL) formation at the plasma membrane under high cholesterol levels and participates in reverse cholesterol transport (PubMed : 25125465). May play a role in the processing of autolysosomes (By similarity).
See full target information ABCA5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 10:20 PubMed38212656

2024

A protective role of ABCA5 in response to elevated sphingomyelin levels in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

YuHong Fu,Russell Pickford,Jasmin Galper,Katherine Phan,Ping Wu,Hongyun Li,Young-Bum Kim,Nicolas Dzamko,Glenda M Halliday,Woojin Scott Kim

Histochemistry and cell biology 155:529-545 PubMed33404706

2021

Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Megan A Palmer,Eleanor Smart,Iain S Haslam

PLoS genetics 10:e1004333 PubMed24831815

2014

Mutations in the cholesterol transporter gene ABCA5 are associated with excessive hair overgrowth.

Applications

IHC-P, ICC/IF, WB

Species

Human, Human, Human

Gina M DeStefano,Mazen Kurban,Kwame Anyane-Yeboa,Claudia Dall'Armi,Gilbert Di Paolo,Heather Feenstra,Nanette Silverberg,Luis Rohena,Larissa D López-Cepeda,Vaidehi Jobanputra,Katherine A Fantauzzo,Maija Kiuru,Marija Tadin-Strapps,Antonio Sobrino,Anna Vitebsky,Dorothy Warburton,Brynn Levy,Julio C Salas-Alanis,Angela M Christiano
View all publications
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