AB230896

Anti-ABCA8 antibody

Name: Anti-ABCA8 antibody (ab230896) | Abcam
Brand: Abcam Limited
SKU: AB230896
Price: 605 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ABCA8 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ABCA8 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0822, ABCA8, ABC-type organic anion transporter ABCA8, ATP-binding cassette sub-family A member 8

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA8 antibody (AB230896)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA8 antibody (AB230896)

Formalin-fixed, paraffin-embedded human brain cortex tissue stained for ABCA8 using ab230896 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ABCA8 antibody (AB230896)

All lanes:

Western blot - Anti-ABCA8 antibody (ab230896) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lane 3:

Rat kidney lysate

Predicted band size: 179 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ABCA8 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O94911

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCA8 also known as ATP-binding cassette sub-family A member 8 is a protein involved in the transport of various molecules across extra- and intracellular membranes. It belongs to the ABC transporter family and has an approximate mass of 160 kDa. The expression of ABCA8 is observed in many tissues with higher levels in brain tissue suggesting it has a significant role in neural function. Researchers often study ABCA8's expression to explore its functions in cellular transport and its impact on cellular homeostasis.

Biological function summary

The protein known as ABCA8 plays an important role in lipid metabolism processes. It participates in the transport of phospholipids and cholesterol contributing to the maintenance of lipid balance within cells. ABCA8 often acts independently not necessitating formation of a larger protein complex. This function plays an important role in the normal functioning of neuron cells highlighting its relevance to brain physiology and metabolism.

Pathways

The activity associated with ABCA8 shows significant involvement in cholesterol metabolism and lipid homeostasis pathways. In these pathways ABCA8 interacts with other proteins including ABCA1 and ABCG1 which also take part in lipid transport activities. These interactions are critical for the regulation of cholesterol efflux and help control how cholesterol moves in and out of cells maintaining cellular lipid homeostasis.

This protein associates with specific conditions affecting lipid metabolism and nervous system function. Notably researchers link ABCA8 with neurological disorders such as Alzheimer's disease due to its expression in the brain and role in cholesterol regulation. Dysregulation of ABCA8 may contribute to the pathogenesis of Alzheimer's disease by affecting cholesterol transport in the brain. Additionally changes in ABCA8 expression or function might play a role in dyslipidemia hinting at its interconnectedness with metabolic proteins like ABCA1 further highlighting its relevance in both neuronal and metabolic health conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Catalyzes ATP-dependent import of organic anions such as taurocholate and estrone sulfate (PubMed : 12379217). In vitro, also imports ochratoxin A (PubMed : 12379217). Also mediates cholesterol efflux independent of apolipoprotein, and plays a role in sphingomyelin production in oligodendrocytes (PubMed : 23560799).. Isoform 3. Catalyzes ATP-dependent efflux of cholesterol and taurocholate (PubMed : 29300488). Interaction with ABCA1 potentiates cholesterol efflux to lipid-free APOA1, which regulates high-density lipoprotein cholesterol levels (PubMed : 28882873).

See full target information ABCA8

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Human reproduction (Oxford, England) 40:119-137 PubMed39521729

2024

Upregulated let-7 expression in the follicular fluid of patients with endometriomas leads to dysfunction of granulosa cells through targeting of IGF1R.

Applications

Unspecified application

Species

Unspecified reactive species

Libing Shi,Hanqi Ying,Yongdong Dai,Yan Rong,Jianmin Chen,Feng Zhou,Shasha Wang,Shiqian Xu,Xiaomei Tong,Songying Zhang

Frontiers in cardiovascular medicine 9:944521 PubMed36312243

2022

Integrated proteomic and metabolomic profile analyses of cardiac valves revealed molecular mechanisms and targets in calcific aortic valve disease.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Fu,Jing Wang,Lianqun Wang,Qiang Wang,Zhigang Guo,Meilin Xu,Nan Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABCA8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA8 antibody (AB230896)

Western blot - Anti-ABCA8 antibody (AB230896)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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