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AB74815

Anti-ABCB10 antibody

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(1 Publication)

Rabbit Polyclonal ABCB10 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ABCB10.
1 Images
Western blot - Anti-ABCB10 antibody (AB74815)
  • WB

Unknown

Western blot - Anti-ABCB10 antibody (AB74815)

All lanes:

Western blot - Anti-ABCB10 antibody (ab74815) at 1/500 dilution

Lane 1:

extracts from COLO cells at 5 µg

Lane 2:

extracts from COLO cells at 5 µg with immunising peptide

Predicted band size: 79 kDa

Observed band size: 116 kDa,60 kDa,79 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ABCB10. The exact immunogen used to generate this antibody is proprietary information.

Q9NRK6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB10 also known as ATP-binding cassette sub-family B member 10 plays a role as a transport protein involved in the mitochondrial export of molecules. It is a member of the ABC (ATP-binding cassette) transporter family characterized by its ability to move various substrates across membranes using ATP hydrolysis. ABCB10 has a molecular mass of approximately 81 kDa and is expressed mainly in the mitochondria of cells with significant levels in tissues with high metabolic activity like the heart and liver.
Biological function summary

ABCB10 contributes to important cellular functions by participating in heme synthesis. It interacts with other mitochondrial proteins forming complexes that facilitate the import of iron and export of heme precursors. This interaction ensures the maintenance of proper heme levels which are necessary for diverse cellular processes including respiration and detoxification pathways. Its activity is not isolated but connected to broader protein networks within the mitochondrial intermembrane space.

Pathways

ABCB10 participates in the heme biosynthesis pathway which is essential for the production of hemoproteins including hemoglobin and cytochromes. Within this context ABCB10 works closely with proteins such as ferrochelatase which catalyzes the final step in heme production. Another related pathway is oxidative phosphorylation where ABCB10 indirectly sustains energy production by ensuring the availability of heme for cytochrome assembly which is pivotal for electron transport chain function.

ABCB10 plays a significant role in conditions related to mitochondrial dysfunction such as sideroblastic anemia and cardiovascular diseases. Mutations affecting ABCB10 can disrupt normal heme synthesis impacting red blood cell production and leading to anemia. Additionally improper function of ABCB10 influences iron homeostasis which associates with proteins like ALAS2 (aminolevulinate synthase 2) further contributing to erythropoietic disorders and highlighting its role in iron-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent transporter located in the mitochondrial inner membrane that catalyzes the export of biliverdin from the mitochondrial matrix, and plays a crucial role in hemoglobin synthesis and antioxidative stress (PubMed : 22085049, PubMed : 28315685, PubMed : 28808058, PubMed : 34011630, PubMed : 37041204). Participates in the early step of the heme biosynthetic process during insertion of iron into protoporphyrin IX (PPIX) (PubMed : 22085049, PubMed : 28808058). Involved in the stabilization of the iron transporter mitoferrin-1/SLC25A37 (By similarity). In addition may be involved in mitochondrial unfolded protein response (UPRmt) signaling pathway, although ABCB10 probably does not participate in peptide export from mitochondria (PubMed : 28315685).
See full target information ABCB10

Alternative Names

ABC-mitochondrial erythroid protein, ATP-binding cassette transporter 10, Mitochondrial ATP-binding cassette 2, ABC-me protein, ABC transporter 10 protein, M-ABC2, ABCB10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 16:18412-38 PubMed26262612

2015

Polymodal Transient Receptor Potential Vanilloid (TRPV) Ion Channels in Chondrogenic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Csilla Szűcs Somogyi,Csaba Matta,Zsofia Foldvari,Tamás Juhász,Éva Katona,Ádám Roland Takács,Tibor Hajdú,Nóra Dobrosi,Pál Gergely,Róza Zákány
View all publications
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Product promise

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