Rabbit Polyclonal ABCB11/BSEP antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Abcb11 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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- Cells 8:2019Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Sheng Chen et. al.PubMed 30884843
- Molecules (Basel, Switzerland) 23:2018Effects of Red Ginseng Extract on the Pharmacokinetics and Elimination of Methotrexate via Mrp2 Regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSowon Lee et. al.PubMed 30424502
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Abcb11 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QY30
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:15791618, PubMed:16332456, PubMed:18985798, PubMed:19228692, PubMed:20010382, PubMed:20398791, PubMed:22262466, PubMed:24711118, PubMed:29507376, PubMed:32203132). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (PubMed:16332456). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (PubMed:15901796, PubMed:18245269).
Alternative names
BSEP, ABCB11, Bile salt export pump, ATP-binding cassette sub-family B member 11
Recommended products
Rabbit Polyclonal ABCB11/BSEP antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Abcb11 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Abcb11 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QY30
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ABCB11 also known as BSEP (bile salt export pump) is a protein that functions as an ATP-binding cassette transporter. It is encoded by the ABCB11 gene and has an approximate molecular mass of 160 kDa. BSEP is mainly expressed in the liver specifically on the canalicular membrane of hepatocytes. It facilitates the flow of bile acids from liver cells into the bile canaliculi playing an important role in bile acid homeostasis and detoxification processes within the liver.
- Biological function summary
BSEP is essential for the transport of bile acids which are important components of bile necessary for the digestion and absorption of lipids and fat-soluble vitamins in the small intestine. BSEP functions as a homodimer and operates by using energy derived from ATP hydrolysis to export bile acids into bile. Malfunction of BSEP can impact bile flow and lead to severe liver conditions as it is the primary bile acid transporter at the canalicular membrane.
- Pathways
BSEP's activity is integral to the enterohepatic circulation of bile acids. It works in conjunction with other transporters such as NTCP (sodium-taurocholate co-transporting polypeptide) and OSTα/β (organic solute transporter alpha-beta). These transporters play roles in the bile acid enterohepatic system contributing to liver-bile-gut circulation. Disruption in BSEP function can alter the FXR (farnesoid X receptor) signaling pathway critical in maintaining the balance of bile acid synthesis and metabolism.
- Associated diseases and disorders
Mutations or functional deficiencies in BSEP are linked to progressive familial intrahepatic cholestasis type 2 (PFIC2) and intrahepatic cholestasis of pregnancy (ICP). These disorders are characterized by impaired bile flow and accumulation of bile acids in the liver. BSEP dysfunction may also interact with other proteins such as MDR3 (multidrug resistance protein 3) which is involved in phospholipid transport and bile formation contributing further to liver pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody (ab217532)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreatic tissue labeling ABCB11/BSEP with ab217532 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody (ab217532)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded human liver carcinoma tissue labeling ABCB11/BSEP with ab217532 at 1/500 dilution overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes and DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH 6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes.
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