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Rabbit Recombinant Monoclonal ABCB11/BSEP antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Transfected cell line, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (AB255605), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (AB255605), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (AB255605), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] (AB255605), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ABCB11/BSEP antibody [EPR22670-64] (AB255605), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IFIPWBIHC-P
Human
Expected
Expected
Not recommended
Not recommended
Tested
Transfected cell line
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line
Dilution info
1/600
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:15791618, PubMed:16332456, PubMed:18985798, PubMed:19228692, PubMed:20010382, PubMed:20398791, PubMed:22262466, PubMed:24711118, PubMed:29507376, PubMed:32203132). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (PubMed:16332456). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (PubMed:15901796, PubMed:18245269).

Alternative names

BSEP, ABCB11, Bile salt export pump, ATP-binding cassette sub-family B member 11

Recommended products

Rabbit Recombinant Monoclonal ABCB11/BSEP antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Transfected cell line, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22670-64
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ABCB11 also known as BSEP (bile salt export pump) is a protein that functions as an ATP-binding cassette transporter. It is encoded by the ABCB11 gene and has an approximate molecular mass of 160 kDa. BSEP is mainly expressed in the liver specifically on the canalicular membrane of hepatocytes. It facilitates the flow of bile acids from liver cells into the bile canaliculi playing an important role in bile acid homeostasis and detoxification processes within the liver.

Biological function summary

BSEP is essential for the transport of bile acids which are important components of bile necessary for the digestion and absorption of lipids and fat-soluble vitamins in the small intestine. BSEP functions as a homodimer and operates by using energy derived from ATP hydrolysis to export bile acids into bile. Malfunction of BSEP can impact bile flow and lead to severe liver conditions as it is the primary bile acid transporter at the canalicular membrane.

Pathways

BSEP's activity is integral to the enterohepatic circulation of bile acids. It works in conjunction with other transporters such as NTCP (sodium-taurocholate co-transporting polypeptide) and OSTα/β (organic solute transporter alpha-beta). These transporters play roles in the bile acid enterohepatic system contributing to liver-bile-gut circulation. Disruption in BSEP function can alter the FXR (farnesoid X receptor) signaling pathway critical in maintaining the balance of bile acid synthesis and metabolism.

Associated diseases and disorders

Mutations or functional deficiencies in BSEP are linked to progressive familial intrahepatic cholestasis type 2 (PFIC2) and intrahepatic cholestasis of pregnancy (ICP). These disorders are characterized by impaired bile flow and accumulation of bile acids in the liver. BSEP dysfunction may also interact with other proteins such as MDR3 (multidrug resistance protein 3) which is involved in phospholipid transport and bile formation contributing further to liver pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BSEP with ab255605 at 1/500 dilution (1.288μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human liver (PMID: 26182153) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling BSEP with ab255605 at 1/ 500 dilution (1.288μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human hepatocellular carcinoma (PMID: 26735860) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinomas tissue labeling BSEP with ab255605 at 1/500 dilution (1.288μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative staining in human cholangiocarcinomas (PMID: 26735860) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling BSEP with ab255605 at 1/100, (6.4μg/ml) dilution, followed by aab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000, (2μg/ml) dilution (Red). Confocal image showing membranous and cytoplasmic staining in 293T cells transfected with myc-tagged BSEP expression vector. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used as a counterstain (Green) at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control: myc-transfected cells.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000, 2μg/ml dilution.

  • Flow Cytometry (Intracellular) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ABCB11/BSEP antibody [EPR22670-64] (ab255605)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with myc-tagged BSEP expression vector cells labeling BSEP with ab255605 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on myc (+) population.

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Product protocols

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