AB256536

Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ABCB11/BSEP antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

View Alternative Names

BSEP, ABCB11, Bile salt export pump, ATP-binding cassette sub-family B member 11

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling BSEP with ab255605 at 1/100, (6.4μg/ml) dilution, followed by ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary antibody at 1/1000, (2μg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in 293T cells transfected with myc-tagged BSEP expression vector. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control : myc-transfected cells.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000, 2μg/ml dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

Flow Cytometry (Intracellular) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with myc-tagged BSEP expression vector cells labeling BSEP with ab255605 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on myc (+) population.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BSEP with ab255605 at 1/500 dilution (1.288μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human liver (PMID : 26182153) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling BSEP with ab255605 at 1/ 500 dilution (1.288μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in canaliculi of human hepatocellular carcinoma (PMID : 26735860) is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Immunohistochemical analysis of paraffin-embedded human cholangiocarcinomas tissue labeling BSEP with ab255605 at 1/500 dilution (1.288μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative staining in human cholangiocarcinomas (PMID : 26735860) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255605).

Unconjugated

Anti-ABCB11/BSEP antibody [EPR22670-64]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-ABCB11/BSEP antibody [EPR22670-64]
578 PE

PE Anti-ABCB11/BSEP antibody [EPR22670-64]
HRP

HRP Anti-ABCB11/BSEP antibody [EPR22670-64]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ABCB11/BSEP antibody [EPR22670-64]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ABCB11/BSEP antibody [EPR22670-64]
660 APC

APC Anti-ABCB11/BSEP antibody [EPR22670-64]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ABCB11/BSEP antibody [EPR22670-64]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ABCB11/BSEP antibody [EPR22670-64]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-ABCB11/BSEP antibody [EPR22670-64]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22670-64

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab256536 is the carrier-free version of ab255605.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB11 also known as BSEP (bile salt export pump) is a protein that functions as an ATP-binding cassette transporter. It is encoded by the ABCB11 gene and has an approximate molecular mass of 160 kDa. BSEP is mainly expressed in the liver specifically on the canalicular membrane of hepatocytes. It facilitates the flow of bile acids from liver cells into the bile canaliculi playing an important role in bile acid homeostasis and detoxification processes within the liver.

Biological function summary

BSEP is essential for the transport of bile acids which are important components of bile necessary for the digestion and absorption of lipids and fat-soluble vitamins in the small intestine. BSEP functions as a homodimer and operates by using energy derived from ATP hydrolysis to export bile acids into bile. Malfunction of BSEP can impact bile flow and lead to severe liver conditions as it is the primary bile acid transporter at the canalicular membrane.

Pathways

BSEP's activity is integral to the enterohepatic circulation of bile acids. It works in conjunction with other transporters such as NTCP (sodium-taurocholate co-transporting polypeptide) and OSTα/β (organic solute transporter alpha-beta). These transporters play roles in the bile acid enterohepatic system contributing to liver-bile-gut circulation. Disruption in BSEP function can alter the FXR (farnesoid X receptor) signaling pathway critical in maintaining the balance of bile acid synthesis and metabolism.

Mutations or functional deficiencies in BSEP are linked to progressive familial intrahepatic cholestasis type 2 (PFIC2) and intrahepatic cholestasis of pregnancy (ICP). These disorders are characterized by impaired bile flow and accumulation of bile acids in the liver. BSEP dysfunction may also interact with other proteins such as MDR3 (multidrug resistance protein 3) which is involved in phospholipid transport and bile formation contributing further to liver pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed : 15791618, PubMed : 16332456, PubMed : 18985798, PubMed : 19228692, PubMed : 20010382, PubMed : 20398791, PubMed : 22262466, PubMed : 24711118, PubMed : 29507376, PubMed : 32203132). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (PubMed : 16332456). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (PubMed : 15901796, PubMed : 18245269).

See full target information ABCB11

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Flow Cytometry (Intracellular) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free (AB256536)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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