Anti-ABCB4 antibody [EPR28774-92]

• WB

Supplier Data

Western blot - Anti-ABCB4 antibody [EPR28774-92] (AB317573)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28220208).

Low expression : brain (PMID : 7734012).

The identity of the bands at 37 kDa and 60 kDa are unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution

Lane 1:

Human liver cancer tissue lysate at 50 µg

Lane 2:

Human liver tissue lysate at 50 µg

Lane 3:

Human brainstem tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 140-250 kDa,36 kDa

true

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-ABCB4 antibody [EPR28774-92] (AB317573)

ABCB4 is a glycoprotein of approximately 150 kDa and detected as a 140-kDa band after treated with Peptide : N-glycosidase F (PNGase F).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28220208, PMID : 24806754).

The identity of the bands at 37 kDa and 60 kDa are unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution

Lane 1:

Untreated Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,150 kDa,36 kDa

true

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-ABCB4 antibody [EPR28774-92] (AB317573)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28220208).

The identity of the bands at 37 kDa and 60 kDa are unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution

All lanes:

McA-RH7777 (rat liver epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140-150 kDa,36 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ABCB4 antibody [EPR28774-92] (AB317573)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28220208).

Low expression : spleen, skeletal muscle (PMID : 7734012).

The identity of the bands at 37 kDa and 60 kDa are unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-E Cadherin antibody [EPR16845-108] (ab181296) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate non-membrane fraction at 20 µg

Lane 2:

Mouse liver tissue lysate membrane fraction at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Lane 7:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140-150 kDa,36 kDa,125 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ABCB4 antibody [EPR28774-92] (AB317573)

This antibody does not cross-react with human ABCB5, ABCB1, ABCB11.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Myc tag® antibody staining at 1/5000 dilution.

All lanes:

Western blot - Anti-ABCB4 antibody [EPR28774-92] (ab317573) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human ABCB4 expression vector containing a His-tag, whole cell lysate at 40 µg

Lane 3:

293T cells transfected with a Human ABCB1 expression vector containing a His-tag, whole cell lysate at 10 µg

Lane 4:

293T cells transfected with a Human ABCB5 expression vector containing a His-tag, whole cell lysate at 40 µg

Lane 5:

293T cells transfected with a Human ABCB11 expression vector containing a His-tag, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 140-250 kDa,36 kDa

false

Exposure time: 37s