AB191058

Anti-ABCB4 antibody - N-terminal

Name: Anti-ABCB4 antibody - N-terminal (ab191058) | Abcam
Brand: Abcam Limited
SKU: AB191058

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(1 Publication)

Rabbit Polyclonal ABCB4 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ABCB4 aa 1-50.

View Alternative Names

MDR3, PGY3, ABCB4, Phosphatidylcholine translocator ABCB4, ATP-binding cassette sub-family B member 4, Multidrug resistance protein 3, P-glycoprotein 3

1 Images

Supplier Data

Western blot - Anti-ABCB4 antibody - N-terminal (AB191058)

All lanes:

Western blot - Anti-ABCB4 antibody - N-terminal (ab191058) at 0.5 µg/mL

All lanes:

MCF7 cell lysate

Predicted band size: 142 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human ABCB4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P21439

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB4 also known as MDR3 is a member of the ATP-binding cassette (ABC) transporter family with a molecular weight of approximately 140 kDa. This protein is mainly expressed in the canalicular membrane of hepatocytes in the liver. Its primary mechanical function involves the translocation of phosphatidylcholine from the inner to the outer leaflet of the hepatocyte membrane into bile.

Biological function summary

This phospholipid translocation is essential for the formation of bile that is non-toxic and aids in digestion. ABCB4 does not function alone; it operates as a part of a heterocomplex with other proteins that contribute to bile formation and secretion. The absence or dysfunction of this target results in imbalanced bile composition which can negatively impact liver function.

Pathways

The ABCB4 protein plays a significant role in the enterohepatic circulation of bile acids. It interacts closely with the bile salt export pump (BSEP) to facilitate the secretion of bile acids into bile. This protein is also linked to the phospholipid transport exact in the hepatic pathways collaborating with other ABC transporters such as ABCB11 underpinning the finely tuned processes that maintain hepatic homeostasis.

Mutations or deficiencies in ABCB4 are commonly associated with progressive familial intrahepatic cholestasis type 3 (PFIC3) and intrahepatic cholestasis of pregnancy (ICP). Defects in the function of this protein lead to toxic bile accumulation causing cholestasis. Other related proteins such as ABCB11 and ATP8B1 may also influence the occurrence of these disorders due to their overlapping roles in hepatobiliary transport processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Isoform 1. Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed : 17523162, PubMed : 21820390, PubMed : 23468132, PubMed : 24594635, PubMed : 24723470, PubMed : 24806754, PubMed : 31873305, PubMed : 7957936, PubMed : 8898203, PubMed : 9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed : 23468132). Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed : 24045840). Promotes biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed : 28012258, PubMed : 9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed : 21820390). Does not confer multidrug resistance (By similarity).

See full target information ABCB4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40004131

2025

Focal Adhesion Kinase Alleviates Simulated Microgravity-Induced Inhibition of Osteoblast Differentiation by Activating Transcriptional Wnt/β-Catenin-BMP2-COL1 and Metabolic SIRT1-PGC-1α-CPT1A Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yiling Bai,Zhaojia Wu,Scot C Leary,Chen Fang,Michelle Yu,Harald Genth,Yufeng Xie,Jinhui Shi,Jim Xiang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ABCB4 antibody - N-terminal

Western blot - Anti-ABCB4 antibody - N-terminal (AB191058)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise