AB24108

Anti-ABCB4 antibody [P3II-26]

Name: Anti-ABCB4 antibody [P3II-26] (ab24108) | Abcam
Brand: Abcam Limited
SKU: AB24108
Price: 445 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal ABCB4 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB4 aa 600-700.

View Alternative Names

MDR3, PGY3, ABCB4, Phosphatidylcholine translocator ABCB4, ATP-binding cassette sub-family B member 4, Multidrug resistance protein 3, P-glycoprotein 3

1 Images

Flow Cytometry - Anti-ABCB4 antibody [P3II-26] (AB24108)

Flow Cyt

Unknown

Flow Cytometry - Anti-ABCB4 antibody [P3II-26] (AB24108)

Overlay histogram showing HeLa cells stained with ab24108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24108, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P3II-26

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human ABCB4 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

P21439

Epitope

Clone P3II-26 reacts with an internal epitope of ABCB4.

Specificity

Clone P3II-26 does not cross-react with the human ABCB1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.1% Sodium azide Constituents: 0.7% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB4 also known as MDR3 is a member of the ATP-binding cassette (ABC) transporter family with a molecular weight of approximately 140 kDa. This protein is mainly expressed in the canalicular membrane of hepatocytes in the liver. Its primary mechanical function involves the translocation of phosphatidylcholine from the inner to the outer leaflet of the hepatocyte membrane into bile.

Biological function summary

This phospholipid translocation is essential for the formation of bile that is non-toxic and aids in digestion. ABCB4 does not function alone; it operates as a part of a heterocomplex with other proteins that contribute to bile formation and secretion. The absence or dysfunction of this target results in imbalanced bile composition which can negatively impact liver function.

Pathways

The ABCB4 protein plays a significant role in the enterohepatic circulation of bile acids. It interacts closely with the bile salt export pump (BSEP) to facilitate the secretion of bile acids into bile. This protein is also linked to the phospholipid transport exact in the hepatic pathways collaborating with other ABC transporters such as ABCB11 underpinning the finely tuned processes that maintain hepatic homeostasis.

Mutations or deficiencies in ABCB4 are commonly associated with progressive familial intrahepatic cholestasis type 3 (PFIC3) and intrahepatic cholestasis of pregnancy (ICP). Defects in the function of this protein lead to toxic bile accumulation causing cholestasis. Other related proteins such as ABCB11 and ATP8B1 may also influence the occurrence of these disorders due to their overlapping roles in hepatobiliary transport processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Isoform 1. Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed : 17523162, PubMed : 21820390, PubMed : 23468132, PubMed : 24594635, PubMed : 24723470, PubMed : 24806754, PubMed : 31873305, PubMed : 7957936, PubMed : 8898203, PubMed : 9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed : 23468132). Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed : 24045840). Promotes biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed : 28012258, PubMed : 9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed : 21820390). Does not confer multidrug resistance (By similarity).

See full target information ABCB4

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:995-1012 PubMed34974814

2022

Prostate-specific membrane antigen modulates the progression of prostate cancer by regulating the synthesis of arginine and proline and the expression of androgen receptors and Fos proto-oncogenes.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Hong,Liang Mao,Luwei Xu,Qiang Hu,Ruipeng Jia

Molecular medicine reports 19:5162-5168 PubMed31059026

2019

Curcumin reverses doxorubicin resistance via inhibition the efflux function of ABCB4 in doxorubicin‑resistant breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjie Wen,Lijuan Fu,Jiafeng Huang,Yi Dai,Bin Wang,Ge Xu,Lanxiang Wu,Honghao Zhou

The Journal of biological chemistry 291:14394 PubMed27371570

2016

Multidrug resistance-associated protein-1 (MRP-1)-dependent glutathione disulfide (GSSG) efflux as a critical survival factor for oxidant-enriched tumorigenic endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gayle M Gordillo,Ayan Biswas,Savita Khanna,James M Spieldenner,Xueliang Pan,Chandan K Sen

The Journal of biological chemistry 291:10089-103 PubMed26961872

2016

Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gayle M Gordillo,Ayan Biswas,Savita Khanna,James M Spieldenner,Xueliang Pan,Chandan K Sen

Toxicological sciences : an official journal of th 145:157-68 PubMed25690737

2015

Comparative Localization and Functional Activity of the Main Hepatobiliary Transporters in HepaRG Cells and Primary Human Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela Bachour-El Azzi,Ahmad Sharanek,Audrey Burban,Ruoya Li,Rémy Le Guével,Ziad Abdel-Razzak,Bruno Stieger,Christiane Guguen-Guillouzo,André Guillouzo

Cancer research 60:5269-77 PubMed11016657

2000

Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

G L Scheffer,M Kool,M Heijn,M de Haas,A C Pijnenborg,J Wijnholds,A van Helvoort,M C de Jong,J H Hooijberg,C A Mol,M van der Linden,J M de Vree,P van der Valk,R P Elferink,P Borst,R J Scheper

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ABCB4 antibody [P3II-26]

Flow Cytometry - Anti-ABCB4 antibody [P3II-26] (AB24108)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise