AB77549

Anti-ABCB5 antibody

Name: Anti-ABCB5 antibody (ab77549) | Abcam
Brand: Abcam Limited
SKU: AB77549
Price: 545 USD
Availability: InStock

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(7 Publications)

Goat Polyclonal ABCB5 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ATP-binding cassette sub-family B member 5 aa 450-500.

View Alternative Names

ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5, ABCB5

2 Images

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Western blot - Anti-ABCB5 antibody (AB77549)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ABCB5 antibody (ab77549) at 0.3 µg/mL

All lanes:

Human skin Lysate in RIPA buffer. at 35 µg

Predicted band size: 90 kDa

Observed band size: 90 kDa

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Unknown

Western blot - Anti-ABCB5 antibody (AB77549)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ABCB5 antibody (ab77549) at 0.03 µg/mL

All lanes:

A431 cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 90 kDa

Observed band size: 80 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human ATP-binding cassette sub-family B member 5 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q2M3G0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab77549 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB5 also known as ATP-binding cassette sub-family B member 5 belongs to the ABC transporter family. This protein functions mechanically as a transmembrane transporter that moves various molecules across cellular membranes using ATP hydrolysis. ABCB5 has a molecular mass of approximately 81 kDa. It is primarily expressed in stem cells melanocytes and certain cancer cells notably in the skin and eye tissues.

Biological function summary

ABCB5 plays an essential role in the regulation of cellular processes including development proliferation and differentiation. It is part of the ATP-binding cassette transporters which help in maintaining cellular homeostasis by exporting toxic substances out of cells. In stem cells ABCB5 maintains the undifferentiated state and protects cells from apoptosis by mediating efflux of drugs contributing to multidrug resistance.

Pathways

ABCB5 interacts with several cellular pathways involved in cell survival and drug resistance. It is closely connected with the Wnt/beta-catenin signaling pathway which regulates cell proliferation and differentiation. ABCB5 also interacts with proteins like P-glycoprotein (ABCB1) as they both participate in similar drug resistance mechanisms affecting therapeutic outcomes in cancer treatment.

Research links ABCB5 to cancer progression and treatment resistance especially in melanoma and colorectal cancer. Its overexpression associates with chemotherapy resistance indicating a need for targeted therapy approaches. In melanoma proteins such as CD133 and ABCB5 together contribute to maintaining cancer stem cell populations which can lead to metastasis and recurrence.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells (PubMed : 12960149, PubMed : 15205344, PubMed : 15899824, PubMed : 22306008). Specifically present in limbal stem cells, where it plays a key role in corneal development and repair (By similarity).

See full target information ATP-binding cassette sub-family B member 5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancer drug resistance (Alhambra, Calif.) 7:29 PubMed39267923

2024

The uniqueness of ABCB5 as a full transporter ABCB5FL and a half-transporter-like ABCB5β.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Gerard,Jean-Pierre Gillet

Pigment cell & melanoma research 33:112-118 PubMed31595650

2019

ABCB5 is activated by MITF and β-catenin and is associated with melanoma differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Pakavarin Louphrasitthiphol,Jagat Chauhan,Colin R Goding

Scientific reports 8:73 PubMed29311580

2018

LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

T Boulding,R D McCuaig,A Tan,K Hardy,F Wu,J Dunn,M Kalimutho,C R Sutton,J K Forwood,A G Bert,G J Goodall,L Malik,D Yip,J E Dahlstrom,A Zafar,K K Khanna,S Rao

Molecular pharmaceutics 11:3452-62 PubMed25115303

2014

Drug resistance is conferred on the model yeast Saccharomyces cerevisiae by expression of full-length melanoma-associated human ATP-binding cassette transporter ABCB5.

Applications

Unspecified application

Species

Unspecified reactive species

Mikhail V Keniya,Ann R Holmes,Masakazu Niimi,Erwin Lamping,Jean-Pierre Gillet,Michael M Gottesman,Richard D Cannon

Cancer research 72:5111-8 PubMed22865455

2012

CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry.

Applications

IHC-P

Species

Human

Chiou-Yan Lai,Brian E Schwartz,Mei-Yu Hsu

The Journal of investigative dermatology 131:944-55 PubMed21270824

2011

RANK is expressed in metastatic melanoma and highly upregulated on melanoma-initiating cells.

Applications

Flow Cyt

Species

Human

Verena Kupas,Carsten Weishaupt,Dorothee Siepmann,Maria-Laura Kaserer,Mareike Eickelmann,Dieter Metze,Thomas A Luger,Stefan Beissert,Karin Loser

Gastroenterology 140:344-55 PubMed20682318

2010

Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance.

Applications

Flow Cyt

Species

Human

Siu Tim Cheung,Phyllis F Y Cheung,Christine K C Cheng,Nicholas C L Wong,Sheung Tat Fan

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ABCB5 antibody

Western blot - Anti-ABCB5 antibody (AB77549)

Western blot - Anti-ABCB5 antibody (AB77549)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise