AB47837

Anti-ABCB6/PRP antibody

Name: Anti-ABCB6/PRP antibody (ab47837) | Abcam
Brand: Abcam Limited
SKU: AB47837
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ABCB6/PRP antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ABCB6 aa 400-500.

View Alternative Names

MTABC3, PRP, UMAT, ABCB6, ATP-binding cassette sub-family B member 6, ABC-type heme transporter ABCB6, Mitochondrial ABC transporter 3, P-glycoprotein-related protein, Ubiquitously-expressed mammalian ABC half transporter, Mt-ABC transporter 3

1 Images

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Western blot - Anti-ABCB6/PRP antibody (AB47837)

All lanes:

Western blot - Anti-ABCB6/PRP antibody (ab47837) at 1/1400 dilution

Lane 1:

KB cell lysate expressing Flag™-tagged human ABCB6/PRP protein

Lane 2:

KB cell lysate without a vector insert

Predicted band size: 94 kDa

Observed band size: 104 kDa,79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

Synthetic Peptide within Human ABCB6 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q9NP58

Specificity

A BLAST analysis was used to suggest cross reactivity with ABCB6 protein from human and dog based on 100% homology with the immunizing sequence. Expect partial reactivity with ABCB6 from mouse and rat sources based on partial (~93%, 15/16) sequence homology. Reactivity against homologues from other sources is not known.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/40000 - 1/130000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This affinity-purified antibody is directed against human ABCB6 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB6 also known as PRP is a transporter protein that plays important roles in cellular processes. This protein is part of the ATP-binding cassette (ABC) transporters specifically the B subfamily which are known for moving various molecules across membranes. ABCB6 has a molecular mass of approximately 92 kDa. It is expressed in various tissues including the liver and erythroid cells indicating its widespread importance in the body.

Biological function summary

The ABCB6 protein is involved in the transport of porphyrins and other tetrapyrroles across membranes. These molecules are important for heme biosynthesis impacting red blood cell production and function. ABCB6 functions as a homodimer and is located on mitochondrial and lysosomal membranes. The transporter's ability to influence heme production links it to essential cellular metabolic processes.

Pathways

The ABCB6 protein is engaged in the heme biosynthesis and mitochondrial function pathways. Its activity supports the synthesis of heme a fundamental component of hemoglobin and other heme-containing proteins. ABCB6 interacts with other proteins such as ALAS2 the erythroid-specific isoform of delta-aminolevulinic acid synthase regulating heme synthesis in erythroid cells. This connection highlights its role in maintaining adequate heme levels within cells.

ABCB6 has been implicated in conditions such as porphyria and anemia. Porphyria is a group of disorders stemming from defects in heme biosynthesis where mutations in ABCB6 may disrupt porphyrin transport aggravating symptoms. Anemia can also arise from impaired ABCB6 function as inadequate heme production affects red blood cell development. In these contexts ABCB6 can connect with proteins like ferrochelatase which plays a role in the final step of heme production illustrating the impact of ABCB6 on disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen (PubMed : 17661442, PubMed : 23792964, PubMed : 27507172, PubMed : 33007128). May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress (PubMed : 10837493, PubMed : 17006453, PubMed : 23792964, PubMed : 33007128). May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils (PubMed : 29940187). In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin (PubMed : 21266531, PubMed : 25202056, PubMed : 31053883). In addition may play a role in the transition metal homeostasis (By similarity).

See full target information ABCB6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:25435 PubMed40659697

2025

A mitochondrial-related gene signature predicts prognosis and immunotherapy response in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongli Shu,Rui Li,Xiaolong Tang,Qiongfang Zhang

American journal of physiology. Renal physiology 319:F654-F663 PubMed32715759

2020

Protective role of DJ-1 in endotoxin-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Leeds,Yogesh Scindia,Valentina Loi,Ewa Wlazlo,Elizabeth Ghias,Sylvia Cechova,Didier Portilla,Jonathan Ledesma,Sundararaman Swaminathan

View all publications

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Product promise

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Anti-ABCB6/PRP antibody

Western blot - Anti-ABCB6/PRP antibody (AB47837)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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