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Rabbit Polyclonal ABCB6/PRP antibody. Suitable for ELISA, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ATP-binding cassette sub-family B member 6, mitochondrial aa 400-500.

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Images

Western blot - Anti-ABCB6/PRP antibody (AB47837), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human ATP-binding cassette sub-family B member 6, mitochondrial aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NP58

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Human
Expected
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Expected
Expected

Species

Human

Dilution info

1/40000 - 1/130000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen (PubMed:17661442, PubMed:23792964, PubMed:27507172, PubMed:33007128). May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress (PubMed:10837493, PubMed:17006453, PubMed:23792964, PubMed:33007128). May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils (PubMed:29940187). In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin (PubMed:21266531, PubMed:25202056, PubMed:31053883). In addition may play a role in the transition metal homeostasis (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal ABCB6/PRP antibody. Suitable for ELISA, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ATP-binding cassette sub-family B member 6, mitochondrial aa 400-500.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human ATP-binding cassette sub-family B member 6, mitochondrial aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NP58
Purification technique

Affinity purification Immunogen

Specificity

A BLAST analysis was used to suggest cross reactivity with ABCB6 protein from human and dog based on 100% homology with the immunizing sequence. Expect partial reactivity with ABCB6 from mouse and rat sources based on partial (~93%, 15/16) sequence homology. Reactivity against homologues from other sources is not known.

Concentration
Loading...
Purification notes

This affinity-purified antibody is directed against human ABCB6 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ABCB6 also known as PRP is a transporter protein that plays important roles in cellular processes. This protein is part of the ATP-binding cassette (ABC) transporters specifically the B subfamily which are known for moving various molecules across membranes. ABCB6 has a molecular mass of approximately 92 kDa. It is expressed in various tissues including the liver and erythroid cells indicating its widespread importance in the body.

Biological function summary

The ABCB6 protein is involved in the transport of porphyrins and other tetrapyrroles across membranes. These molecules are important for heme biosynthesis impacting red blood cell production and function. ABCB6 functions as a homodimer and is located on mitochondrial and lysosomal membranes. The transporter's ability to influence heme production links it to essential cellular metabolic processes.

Pathways

The ABCB6 protein is engaged in the heme biosynthesis and mitochondrial function pathways. Its activity supports the synthesis of heme a fundamental component of hemoglobin and other heme-containing proteins. ABCB6 interacts with other proteins such as ALAS2 the erythroid-specific isoform of delta-aminolevulinic acid synthase regulating heme synthesis in erythroid cells. This connection highlights its role in maintaining adequate heme levels within cells.

Associated diseases and disorders

ABCB6 has been implicated in conditions such as porphyria and anemia. Porphyria is a group of disorders stemming from defects in heme biosynthesis where mutations in ABCB6 may disrupt porphyrin transport aggravating symptoms. Anemia can also arise from impaired ABCB6 function as inadequate heme production affects red blood cell development. In these contexts ABCB6 can connect with proteins like ferrochelatase which plays a role in the final step of heme production illustrating the impact of ABCB6 on disease progression.

Product promise

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1 product image

  • Western blot - Anti-ABCB6/PRP antibody (ab47837), expandable thumbnail

    Western blot - Anti-ABCB6/PRP antibody (ab47837)

    All lanes: Western blot - Anti-ABCB6/PRP antibody (ab47837) at 1/1400 dilution

    Lane 1: KB cell lysate expressing Flag™-tagged human ABCB6/PRP protein

    Lane 2: KB cell lysate without a vector insert

    Predicted band size: 94 kDa

    Observed band size: 104 kDa, 79 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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