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AB194409

Anti-ABCB6/PRP antibody - C-terminal

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(1 Publication)

Mouse Polyclonal ABCB6/PRP antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB6 aa 700 to C-terminus.

View Alternative Names

MTABC3, PRP, UMAT, ABCB6, ATP-binding cassette sub-family B member 6, ABC-type heme transporter ABCB6, Mitochondrial ABC transporter 3, P-glycoprotein-related protein, Ubiquitously-expressed mammalian ABC half transporter, Mt-ABC transporter 3

4 Images
Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)
  • WB

Supplier Data

Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)

All lanes:

Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution

All lanes:

RAW264.7 cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 94 kDa

false

Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)
  • WB

Supplier Data

Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)

All lanes:

Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution

All lanes:

PC-12 cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 94 kDa

false

Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)
  • WB

Supplier Data

Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)

All lanes:

Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution

All lanes:

NIH3T3 cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 94 kDa

false

Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)
  • WB

Supplier Data

Western blot - Anti-ABCB6/PRP antibody - C-terminal (AB194409)

All lanes:

Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 94 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ABCB6 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NP58

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB6 also known as PRP is a transporter protein that plays important roles in cellular processes. This protein is part of the ATP-binding cassette (ABC) transporters specifically the B subfamily which are known for moving various molecules across membranes. ABCB6 has a molecular mass of approximately 92 kDa. It is expressed in various tissues including the liver and erythroid cells indicating its widespread importance in the body.
Biological function summary

The ABCB6 protein is involved in the transport of porphyrins and other tetrapyrroles across membranes. These molecules are important for heme biosynthesis impacting red blood cell production and function. ABCB6 functions as a homodimer and is located on mitochondrial and lysosomal membranes. The transporter's ability to influence heme production links it to essential cellular metabolic processes.

Pathways

The ABCB6 protein is engaged in the heme biosynthesis and mitochondrial function pathways. Its activity supports the synthesis of heme a fundamental component of hemoglobin and other heme-containing proteins. ABCB6 interacts with other proteins such as ALAS2 the erythroid-specific isoform of delta-aminolevulinic acid synthase regulating heme synthesis in erythroid cells. This connection highlights its role in maintaining adequate heme levels within cells.

ABCB6 has been implicated in conditions such as porphyria and anemia. Porphyria is a group of disorders stemming from defects in heme biosynthesis where mutations in ABCB6 may disrupt porphyrin transport aggravating symptoms. Anemia can also arise from impaired ABCB6 function as inadequate heme production affects red blood cell development. In these contexts ABCB6 can connect with proteins like ferrochelatase which plays a role in the final step of heme production illustrating the impact of ABCB6 on disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen (PubMed : 17661442, PubMed : 23792964, PubMed : 27507172, PubMed : 33007128). May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress (PubMed : 10837493, PubMed : 17006453, PubMed : 23792964, PubMed : 33007128). May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils (PubMed : 29940187). In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin (PubMed : 21266531, PubMed : 25202056, PubMed : 31053883). In addition may play a role in the transition metal homeostasis (By similarity).
See full target information ABCB6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mitochondrion 45:52-62 PubMed29474837

2018

Mitochondrial accumulation of doxorubicin in cardiac and diaphragm muscle following exercise preconditioning.

Applications

WB

Species

Rat

Aaron B Morton,Andres Mor Huertas,J Matthew Hinkley,Noriko Ichinoseki-Sekine,Demetra D Christou,Ashley J Smuder
View all publications
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Product promise

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