Mouse Polyclonal ABCB6/PRP antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB6 aa 700 to C-terminus.
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- Mitochondrion 45:52-622018Mitochondrial accumulation of doxorubicin in cardiac and diaphragm muscle following exercise preconditioning.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ABCB6 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NP58
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2500 | Notes - |
Species Rat | Dilution info 1/500 - 1/2500 | Notes - |
Species Human | Dilution info 1/500 - 1/2500 | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen (PubMed:17661442, PubMed:23792964, PubMed:27507172, PubMed:33007128). May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress (PubMed:10837493, PubMed:17006453, PubMed:23792964, PubMed:33007128). May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils (PubMed:29940187). In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin (PubMed:21266531, PubMed:25202056, PubMed:31053883). In addition may play a role in the transition metal homeostasis (By similarity).
Alternative names
MTABC3, PRP, UMAT, ABCB6, ATP-binding cassette sub-family B member 6, ABC-type heme transporter ABCB6, Mitochondrial ABC transporter 3, P-glycoprotein-related protein, Ubiquitously-expressed mammalian ABC half transporter, Mt-ABC transporter 3
Recommended products
Mouse Polyclonal ABCB6/PRP antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB6 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ABCB6 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NP58
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ABCB6 also known as PRP is a transporter protein that plays important roles in cellular processes. This protein is part of the ATP-binding cassette (ABC) transporters specifically the B subfamily which are known for moving various molecules across membranes. ABCB6 has a molecular mass of approximately 92 kDa. It is expressed in various tissues including the liver and erythroid cells indicating its widespread importance in the body.
- Biological function summary
The ABCB6 protein is involved in the transport of porphyrins and other tetrapyrroles across membranes. These molecules are important for heme biosynthesis impacting red blood cell production and function. ABCB6 functions as a homodimer and is located on mitochondrial and lysosomal membranes. The transporter's ability to influence heme production links it to essential cellular metabolic processes.
- Pathways
The ABCB6 protein is engaged in the heme biosynthesis and mitochondrial function pathways. Its activity supports the synthesis of heme a fundamental component of hemoglobin and other heme-containing proteins. ABCB6 interacts with other proteins such as ALAS2 the erythroid-specific isoform of delta-aminolevulinic acid synthase regulating heme synthesis in erythroid cells. This connection highlights its role in maintaining adequate heme levels within cells.
- Associated diseases and disorders
ABCB6 has been implicated in conditions such as porphyria and anemia. Porphyria is a group of disorders stemming from defects in heme biosynthesis where mutations in ABCB6 may disrupt porphyrin transport aggravating symptoms. Anemia can also arise from impaired ABCB6 function as inadequate heme production affects red blood cell development. In these contexts ABCB6 can connect with proteins like ferrochelatase which plays a role in the final step of heme production illustrating the impact of ABCB6 on disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409)
All lanes: Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution
All lanes: NIH3T3 cell lysate at 50 µg
SecondaryAll lanes: Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 94 kDa
Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409)
All lanes: Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution
All lanes: RAW264.7 cell lysate at 50 µg
SecondaryAll lanes: Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 94 kDa
Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409)
All lanes: Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution
All lanes: PC-12 cell lysate at 50 µg
SecondaryAll lanes: Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 94 kDa
Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409)
All lanes: Western blot - Anti-ABCB6/PRP antibody - C-terminal (ab194409) at 1/500 dilution
All lanes: HeLa cell lysate at 50 µg
SecondaryAll lanes: Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 94 kDa
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